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DNTP100

Sigma-Aldrich

Deoxynucleotide Set, 100 mM

Individual dNTPs for routine PCR; 0.25 mL each

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Synonym(s):
DNTP Set for PCR
NACRES:
NA.52

form

liquid

concentration

100 mM

color

colorless

shipped in

dry ice

storage temp.

−20°C

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This Item
DNTP10DNTP100AD7295
concentration

100 mM

concentration

10 mM

concentration

100 mM

concentration

10 mM

color

colorless

color

colorless

color

colorless

color

colorless

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

300

General description

Deoxynucleotide triphosphates (dNTPs) are organic molecules, which contains nucleobase bound to deoxyribose. This dNTPs set includes 0.25 mL each of 100mM aqueous solutions of four deoxyribonucleotides, deoxyribose adenosine triphosphate (dATP), deoxyribose cytidine triphosphate (dCTP), deoxyribose guanosine triphosphate (dGTP), and deoxyribose thymidine triphosphate (dTTP) in separate tubes for convenience and easy PCR reaction setup.

Application

Deoxynucleotide Set, 100 mM has been used as a component of the reaction mixture for polymerase chain reaction (PCR).It has also been used as a component of the reaction mixture for amplification refractory mutation system polymerase chain reaction (ARMS-PCR) to genotype single-nucleotide polymorphisms (SNPs).
Deoxynucleotide Set, 100 mM has been used:
  • to biotinylate λ-DNA for magnetic tweezers measurements
  • in RAPD PCR
  • in FISH probe generation and labeling
  • in PCR amplification of genomic DNA
  • in multiplex PCR
  • in first-strand cDNA synthesis
  • in TUNEL labeling
  • in RT-PCR
  • as a component of the reaction mixture for polymerase chain reaction (PCR)

Biochem/physiol Actions

Deoxynucleotide triphosphates (dNTPs) are substrates for DNA polymerizing enzymes. They act as building blocks of nucleic acid molecules and are acidic in nature. dNTPs are key components of PCR master mixes and are highly essential to amplify DNA. Optimal levels of dNTPs ensure proper chromosomal replication and in turn maintain genome stability.

Features and Benefits

  • Provided as 4 separate tubes of dNTPs.
  • Allows optimization of individual dNTP concentrations.

Packaging

0.25 mL each of 100 mM dATP, dCTP, dGTP and dTTP

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Expression of Mx gene in Cirrhinus mrigala (Hamilton, 1822) to OmpC protein of Aeromonas hydrophila and bacterial infection
Roy P, et al.
Applied Biochemistry and Biotechnology, 178, 640-653 (2016)
A critical balance: dNTPs and the maintenance of genome stability
<BIG><BIG>Pai CC and Kearsey SE</BIG></BIG>
Genes, 8, 57-57 (2017)
Attractive forces between cation condensed DNA double helices
Todd BA, et al.
Biophysical Journal, 94, 4775-4782 (2008)
Multiple locus VNTR fingerprinting (MLVF) of Streptococcus pyogenes
Obszanska K, et al.
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