The refractive index of the Duolink® In Situ Mounting Medium with DAPI is 1.461. Compatibility with Total Internal Reflection Fluorescence (TIRF) Microscopy has not been tested in-house.
DUO82040
Duolink® In Situ Mounting Medium with DAPI
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 5 mL | Check Cart for Availability | $290.00 |
About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
product line
Duolink®
Quality Level
technique(s)
proximity ligation assay: suitable
fluorescence
λex 360 nm; λem 460 nm (Zeiss Filter set 49)
suitability
suitable for fluorescence
shipped in
wet ice
storage temp.
2-8°C
Application
Duolink® proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.
Use the Duolink® In Situ Fluorescence Protocol for this product. A set of short instructionsis also available.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink®PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Use the Duolink® In Situ Fluorescence Protocol for this product. A set of short instructionsis also available.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink®PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Specificity
Duolink® In Situ Mounting Medium with DAPI is ideal for nuclear staining and preserving signals generated with the Duolink® In Situ Detection Reagents for fluorescence microscopy. See datasheet for more details.
Note: Counterstaining with Cy®2 is not recommended.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects
View full Duolink® product list
Duolink® In Situ Mounting Medium with DAPI is ideal for nuclear staining and preserving signals generated with the Duolink® In Situ Detection Reagents for fluorescence microscopy. See datasheet for more details.
Note: Counterstaining with Cy®2 is not recommended.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects
View full Duolink® product list
Duolink® In Situ mounting medium with DAPI has been used to mount coverslips in proximity ligation assay (PLA) for nuclear staining.
Features and Benefits
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Single molecule sensitivity due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Quicker and simpler than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
Legal Information
Cy is a registered trademark of Cytiva
Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany
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This Item | |||
|---|---|---|---|
| technique(s) proximity ligation assay: suitable | technique(s) immunofluorescence: suitable, proximity ligation assay: suitable | technique(s) immunofluorescence: suitable, proximity ligation assay: suitable | technique(s) proximity ligation assay: suitable |
| suitability suitable for fluorescence | suitability suitable for brightfield, suitable for fluorescence | suitability suitable for brightfield, suitable for fluorescence | suitability suitable for fluorescence-detection automated sequencing, suitable for microtiter plates |
| fluorescence λex 360 nm; λem 460 nm (Zeiss Filter set 49) | fluorescence - | fluorescence - | fluorescence λex 360 nm; λem 460 nm |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in dry ice |
signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 3 - Met. Corr. 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Storage Class
10 - Combustible liquids
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Protocols
This protocol describes how to perform immunofluorescent detection of proteins in cells and tissue.
Duolink® PLA Multicolor Detection Protocol
This page details the Duolink® In Situ Short Protocol for fluorescence detection
Articles
Support information including tips and tricks, frequently asked questions, and basic troubleshooting.
Learn how Proximity Ligation Assay technology works and how the protein-protein interaction control kit can confirm in situ detection of EGF-induced EGFR-HER2 dimerization.
Proteins interact with various molecules, including other proteins, to fulfill complex cellular functions within biological systems.
Related Content
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Applications to detect, quantify and visualize protein-protein interactions, post-translational modifications and low expression protein detection using proximity ligation assay
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Current Protocols in Pharmacology / Editorial Board, S.J. Enna (editor-in-chief) ... [Et al.], 2-16 (2016)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| DUO82040-5ML | 04061833591888 |
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What is the refractive index of the Duolink® In Situ Mounting Medium with DAPI (DUO82040)? Is it compatible with Total Internal Reflection Fluorescence (TIRF) Microscopy?
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