DUO92102

Sigma-Aldrich

Duolink® In Situ Orange Starter Kit Mouse/Rabbit

NACRES:
NA.32

product line

Duolink®

application(s)

proximity ligation assay: suitable

fluorescence

λex 554 nm; λem 576 nm (orange) (Cyanine 3; Zeiss Filter set 20)

suitability

suitable for fluorescence

storage temp.

−20°C

Application

Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Follow the
Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.

Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.

To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes.This starter kit supplies all other necessary reagents for 30 Duolink® PLA reactions, which include a pair of PLA probes (Anti-Rabbit PLUS and Anti-Mouse MINUS), orange detection reagents, wash buffers, and mounting medium.Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.

Specificity
The Duolink® In Situ Orange Starter Kit Mouse/Rabbit requires one primary antibody from mouse and one primary antibody from rabbit. Orange fluorescence detection reagents are often used with Cyanine 3 filter.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects

View full Duolink® product list
Duolink® In Situ Orange Starter Kit Mouse/Rabbit has been used in in situ interaction assay.

Features and Benefits

  • No overexpression or genetic manipulation required
  • High specificity (fewer false positives)
  • Single molecule sensitivity due to rolling circle amplification
  • Relative quantification possible
  • No special equipment needed
  • Quicker and simpler than FRET
  • Increased accuracy compared to co-IP
  • Publication-ready results

Legal Information

Duolink is a registered trademark of Sigma-Aldrich Co. LLC
PLA is a registered trademark of Sigma-Aldrich Co. LLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Kit Components Also Available Separately

Product No.
Description
SDS

  • DUO92002Duolink® In Situ PLA® Probe Anti-Rabbit PLUS, Affinity purified Donkey anti-Rabbit IgG (H+L)

  • DUO92004Duolink® In Situ PLA® Probe Anti-Mouse MINUS, Affinity purified Donkey anti-Mouse IgG (H+L)

  • DUO92007Duolink® In Situ Detection Reagents Orange

  • DUO82049Duolink® In Situ Wash Buffers, Fluorescence

  • DUO82040Duolink® In Situ Mounting Medium with DAPI

RIDADR

NONH for all modes of transport

Certificate of Analysis

Certificate of Origin

Interactome analysis reveals ZNF804A, a schizophrenia risk gene, as a novel component of protein translational machinery critical for embryonic neurodevelopment
Zhou Y, et al.
Molecular Psychiatry (2017)
Tomokazu Sumida et al.
Nature immunology (2018-10-31)
Foxp3+ regulatory T cells (Treg cells) are the central component of peripheral immune tolerance. Whereas a dysregulated Treg cytokine signature has been observed in autoimmune diseases, the regulatory mechanisms underlying pro- and anti-inflammatory cytokine production are elusive. Here, we identify...
Roberto Di Maio et al.
Science translational medicine, 10(451) (2018-07-27)
Missense mutations in leucine-rich repeat kinase 2 (LRRK2) cause familial Parkinson's disease (PD). However, a potential role of wild-type LRRK2 in idiopathic PD (iPD) remains unclear. Here, we developed proximity ligation assays to assess Ser1292 phosphorylation of LRRK2 and, separately...
Peng Jiang et al.
PloS one, 10(6), e0128744-e0128744 (2015-06-09)
Exposure of platelets to collagen triggers the formation of a platelet clot. Pharmacological agents capable of inhibiting platelet activation by collagen are thus of potential therapeutic interest. Thrombus formation is initiated by the interaction of the GPIb-V-IX complex with collagen-bound...
Xiaodong Xu et al.
Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology, 49(11), 677-681 (2015-01-28)
To evaluate and compare the physical and chemical properties of four different dental cements under long-term water storage. A glass-ionomer cement (A:Fuji I), a resin reinforced glass-ionomer (B: Fuji Plus), a self-adhesive resin cement (C:G-Cem), and an etch & rinse...
Articles
Learn how Proximity Ligation Assay technology works and how the protein-protein interaction control kit can confirm in situ detection of EGF-induced EGFR-HER2 dimerization.
Read More
Duolink® proximity ligation assay used to study neuron interactions furthering neuroscience research.
Read More
Protocol for immunofluorescent detection of proteins in cells and tissue
Read More
Things to consider for preparation, setup and execution of the Duolink® assay protocol
Read More
Protocols
This page details the Duolink® In Situ Short Protocol for fluorescence detection
Read More
Related Content
Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.
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Applications to detect, quantify and visualize protein-protein interactions, post-translational modifications and low expression protein detection using proximity ligation assay
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