DUO92103

Sigma-Aldrich

Duolink® In Situ Red Starter Kit Mouse/Goat

NACRES:
NA.32

Quality Level

product line

Duolink®

application(s)

proximity ligation assay: suitable

fluorescence

λex 594 nm; λem 624 nm (red) (Texas Red®; Zeiss Filter set 31)

suitability

suitable for fluorescence

storage temp.

−20°C

Application

Duolink® In Situ Red Starter Kit Mouse/Goat has been used in the critical commercial assay and proximity ligation assay (PLA).
Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.

Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.

To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. This starter kit supplies all other necessary reagents for 30 Duolink® PLA reactions, which include a pair of PLA probes (Anti-Mouse PLUS and Anti-Goat MINUS), red detection reagents, wash buffers, and mounting medium.Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.

Specificity
The Duolink® In Situ Red Starter Kit Mouse/Goat requires one primary antibody from mouse and one primary antibody from goat. Red fluorescence detection reagents are often used with Texas Red® filter.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink®PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects

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Features and Benefits

  • No overexpression or genetic manipulation required
  • High specificity (fewer false positives)
  • Single molecule sensitivity due to rolling circle amplification
  • Relative quantification possible
  • No special equipment needed
  • Quicker and simpler than FRET
  • Increased accuracy compared to co-IP
  • Publication-ready results

Legal Information

Duolink is a registered trademark of Sigma-Aldrich Co. LLC
PLA is a registered trademark of Sigma-Aldrich Co. LLC
Texas Red is a registered trademark of Life Technologies

Kit Components Also Available Separately

Product No.
Description
SDS

  • DUO92006Duolink® In Situ PLA® Probe Anti-Goat MINUS, Affinity purified Donkey anti-Goat IgG (H+L)

  • DUO92001Duolink® In Situ PLA® Probe Anti-Mouse PLUS

  • DUO92008Duolink® In Situ Detection Reagents Red

  • DUO82049Duolink® In Situ Wash Buffers, Fluorescence

  • DUO82040Duolink® In Situ Mounting Medium with DAPI

Signal Word

Warning

Hazard Statements

hazcat

Aquatic Chronic 2 - Met. Corr. 1 - Skin Sens. 1

storage_class_code

8A - Combustible, corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

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Articles
Duolink® proximity ligation assay used to study neuron interactions furthering neuroscience research.
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Protocol for immunofluorescent detection of proteins in cells and tissue
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Things to consider for preparation, setup and execution of the Duolink® assay protocol
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Protocols
This page details the Duolink® In Situ Short Protocol for fluorescence detection
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