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E0276

Sigma-Aldrich

EPPS

BioPerformance Certified, suitable for cell culture, ≥99.5% (titration)

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Synonym(s):
4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic acid, 4-(2-Hydroxyethyl)piperazine-1-propanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(3-propanesulfonic acid), HEPPS
Empirical Formula (Hill Notation):
C9H20N2O4S
CAS Number:
Molecular Weight:
252.33
Beilstein:
3957385
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

BioPerformance Certified

Assay

≥99.5% (titration)

form

crystalline powder

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin and Total Aerobic Microbial Count, tested

pH

7.3-8.7

useful pH range

7.3-8.7

pKa (25 °C)

8.0

pKa 

8

mp

237-239 °C (lit.)

solubility

H2O: 25 g + 50 mL, clear, colorless

cation traces

heavy metals (as Pb): ≤5 ppm

λmax

A290 at ≤0.1 (C=33% w/w)

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CCCS(O)(=O)=O)CC1

InChI

1S/C9H20N2O4S/c12-8-7-11-5-3-10(4-6-11)2-1-9-16(13,14)15/h12H,1-9H2,(H,13,14,15)

InChI key

OWXMKDGYPWMGEB-UHFFFAOYSA-N

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This Item
E1894E9502H3784
EPPS BioPerformance Certified, suitable for cell culture, ≥99.5% (titration)

Sigma-Aldrich

E0276

EPPS

Premium Grade
EPPS BioXtra, ≥99.5% (titration)

Sigma-Aldrich

E1894

EPPS

Premium Grade
EPPS ≥99.5% (titration)

Sigma-Aldrich

E9502

EPPS

-
HEPES sodium salt BioPerformance Certified, suitable for cell culture, ≥99.0%

Sigma-Aldrich

H3784

HEPES sodium salt

Premium Grade
form

crystalline powder

form

powder

form

powder

form

powder

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin and Total Aerobic Microbial Count, tested

impurities

≤0.005% Phosphorus (P), ≤0.1% Insoluble matter

impurities

-

impurities

endotoxin and total aerobic microbial count, tested

pH

7.3-8.7

pH

5.0-7.0 (20 °C, 1 M in H2O)

pH

7.3-8.7

pH

10-12 (1 M in water)

solubility

H2O: 25 g + 50 mL, clear, colorless

solubility

H2O: 1 M at 20 °C, clear, colorless

solubility

H2O: 25 g + 50 mL, clear, colorless

solubility

water: 0.1 M, clear, colorless

General description

EPPS or 4-(2-hydroxyethyl)piperazine-1-propanesulfonic acid (HEPPS) is a zwitterionic buffer substance from the group of good buffers. It effectively maintains constant pH in a solution, which is necessary for many biological and biochemical reactions. EPPS buffer is widely used in cell culture experiments and biological research.

Application

EPPS may be used as a buffer solution to stabilize the pH in sea water samples during the titration experiments.

Features and Benefits

  • BioPerformance Certified, high-quality level (400), ensures reliable and consistent results.
  • Suitable for mammalian cell culture techniques

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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The characteristics of Fe speciation and Fe-binding ligands in the Mariana back-arc hydrothermal plumes
Wang H R, et al.
Geochimica et Cosmochimica Acta, 292, 24-36 (2021)
lonization Enthalpies of Some Common Zvvitterionic Hydrogen-lon Buffers (HEPES, PIPES, HEPPS and BES) for Biological Research
Roig T, et al.
Journal of Separation Science, 993(47), 899-90j-899-90j (1993)
Equilibrium studies, synthesis, and characterization of zwitterionic buffer (HEPES and HEPPS) dimethyltin (IV) complexes
Mahmoud M, et al.
Main Group Chemistry, 6(2) (2007)
Feng Xie et al.
Cancer letters, 364(2), 106-117 (2015-05-17)
Cervical cancer is often associated with eosinophil (EOS) infiltration, but the source and the role of EOS are still largely unknown. Our previous work has established that thymic stromal lymphopoietin (TSLP) can stimulate the growth of cervical cancer cell in
Xiannu Jin et al.
Journal of pharmacological and toxicological methods, 70(2), 188-194 (2014-08-26)
Malaria is a major health concern and affects over 300million people a year. Accordingly, there is an urgent need for new efficacious anti-malarial drugs. A major challenge in developing new anti-malarial drugs is to design active molecules that have preferable

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