MilliporeSigma
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E0532

Sigma-Aldrich

Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse

clone 102618, purified immunoglobulin, lyophilized powder

Synonym(s):
Anti-EGFR
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

102618, monoclonal

form

lyophilized powder

species reactivity

human

technique(s)

western blot: 1-2 μg/mL

isotype

IgG2b

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... EGFR(1956)

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General description

The members of epidermal growth factor receptor (EGF R) or the ErbB receptor family have been identified as useful biomarkers and targets for cancer therapy. The EGFR family includes four receptor tyrosine kinases, EGF R (ErbB1), ErbB2 (neu), ErbB3, and ErbB4. EGF R binds EGF and induces tyrosine phosphorylation leading to proliferation of cells. EGF R is present on many cell types of epithelial and mesenchymal lineages. EGF R is capable of binding transforming growth factor-α and heparin-binding EGF in addition to EGF. There are numerous effector molecule activated by EGF R that result in a variety of biological processes such as morphogenesis, cell motility, apoptosis, differentiation and organ repair and maintenance. Deregulation of EGF R signaling is implicated in progression of a wide variety of tumors, invasion and metastasis
Monoclonal anti-EGF Receptor detects human EGF receptor. This antibody shows no cross-reactivity with recombinant mouse EGF R, recombinant human ErbB2, recombinant mouse ErbB2, recombinant human ErbB3, or recombinant human ErbB4.

Immunogen

purified, NSO cell-derived recombinant human epidermal growth factor receptor extracellular domain.

Application

Anti-EGF R antibody may be used at a working concentration of 1-2 μg/ml for detection of human EGF R by immunoblotting.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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