MilliporeSigma
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E0887

Sigma-Aldrich

Esterase from rabbit liver

lyophilized powder, ≥30 units/mg protein

Synonym(s):
Carboxyl esterase, Carboxylic-ester hydrolase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

form

lyophilized powder

Quality Level

specific activity

≥30 units/mg protein

mol wt

60 kDa

composition

Protein, ≥85% biuret

storage temp.

−20°C

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General description

Esterase belongs to the C-family of carboxylesterases. It is a glycoprotein, mainly present in the endoplasmic reticulum of hepatocytes.

Application

Esterase from rabbit liver has been used in a study to investigate a toxic effect of carbamate insecticides. Esterase from rabbit liver has also been used in a study to investigate the effect of simvastatin on expression and activity of a lipoprotein-associated phospholipase A(2).
Esterase from rabbit liver has been used:
  • in comparative biophysical studies with bovine bile-salt-activated lipase in the assays with vinyl acetate, triacylglycerols, p-nitrophenyl esters and serine enzyme inhibitors
  • in the catalysis and kinetic profiling studies of peptidic compounds
  • in in vitro stability assays of prodrug compounds (BMS-292655 and BMS300043)
  • in the hydrolysis of oleuropein aglycone (Ole-AG)

The enzyme from Sigma has been used to study the effect of divalent metal ions on the activity of esterase.

Packaging

500, 1000 units in poly bottle

Biochem/physiol Actions

Esterase enzyme catalyzes hydrolysis of carboxylic ester to carboxylate and alcohol in the presence of water. Rabbit liver carboxylesterase catalyzes enantioselective hydrolysis of cineol derivatives.
Esterase enzyme has broad substrate specificity. Esterase from rabbit liver has been used to investigate the toxic effect of carbamate insecticides. Esterase from rabbit liver has also been used for the hydrolysis of simvastatin.

Unit Definition

One unit will hydrolyze 1.0 μmole of ethyl butyrate to butyric acid and ethanol per min at pH 8.0 at 25 °C.

Physical form

Lyophilized powder containing Tris buffer salts

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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