cell culture | mammalian: suitable
12 - Non Combustible Liquids
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
ECM gel can be diluted twofold with cold Dulbecco's Modified Eagle's Medium (DME). This means 1 part ECM gel and 1 part DME.
Product No. E1270, ECM gel is the equivalent of Matrigel from BD only produced by Sigma-Aldrich.
Product No. E1270 is not growth factor reduced. Product No. E6909 is ECM gel, growth factor reduced.
Product E1270 ECM gel does contain phenol-red from the Dulbecco's modified Eagle's medium component of the product. Product No. E6909, ECM gel, growth factor reduced, is phenol red free.
The approximate concentration of CaCl2 in the ECM solution is 0.265 mg/mL.
Dispase (Product No. D4818) is a Bacillus-derived neutral protease that is recommended for recovering cells cultured on ECM (Product No. E1270) or Matrigel. Dispase will yield a single cell suspension far more effectively than trypsin, collagenase, or other proteolytic enzymes. Dispase is gentle and will not damage cells harvested for sub-cultivation or other study.
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.