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MilliporeSigma

E1385

Sigma-Aldrich

Ethidium bromide solution

BioReagent, Molecular Biology, 500 μg/mL in H2O

Synonym(s):

3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide, EtBr, Homidium bromide

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About This Item

Empirical Formula (Hill Notation):
C21H20BrN3
CAS Number:
1239-45-8
Molecular Weight:
394.31
Beilstein/REAXYS Number:
3642536
MDL number:
MFCD00011724
UNSPSC Code:
41105322
PubChem Substance ID:
24894408
NACRES:
NA.31

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grade

Molecular Biology

Quality Level

200

product line

BioReagent

concentration

500 μg/mL in H2O

technique(s)

electrophoresis: suitable

suitability

suitable for gel electrophoresis

SMILES string

[Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

InChI

1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H

InChI key

ZMMJGEGLRURXTF-UHFFFAOYSA-N

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This Item
46067E7637E8751
Ethidium bromide solution BioReagent, Molecular Biology, 500 μg/mL in H2O

E1385

Ethidium bromide solution

Ethidium bromide solution suitable for fluorescence, ~1% in H2O

46067

Ethidium bromide solution

Ethidium bromide BioReagent, Molecular Biology, powder

E7637

Ethidium bromide

Ethidium bromide ~95% (HPLC)

E8751

Ethidium bromide

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

electrophoresis: suitable

technique(s)

-

grade

for molecular biology

grade

for fluorescence

grade

for molecular biology

grade

-

suitability

suitable for gel electrophoresis

suitability

-

suitability

suitable for gel electrophoresis

suitability

-

concentration

500 μg/mL in H2O

concentration

~1% in H2O

concentration

-

concentration

-

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

product line

BioReagent

product line

-

product line

BioReagent

product line

-

Application

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.
Ethidium bromide solution has been used to deplete mtDNA to investigate mitochondrial damage signals in autophagy.[1] It has also been used to detect DNA damage using comet assay.[2]

Biochem/physiol Actions

Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

Reconstitution

For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SHBR2368
SHBR0113
SHBQ1937
SHBN4521
MKBV9660V

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Questions

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  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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