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E1385

Sigma-Aldrich

Ethidium bromide solution

BioReagent, for molecular biology, 500 μg/mL in H2O

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Synonym(s):
3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide, EtBr, Homidium bromide
Empirical Formula (Hill Notation):
C21H20BrN3
CAS Number:
Molecular Weight:
394.31
Beilstein:
3642536
MDL number:
PubChem Substance ID:
NACRES:
NA.31

grade

for molecular biology

product line

BioReagent

concentration

500 μg/mL in H2O

technique(s)

electrophoresis: suitable

suitability

suitable for gel electrophoresis

SMILES string

[Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

InChI

1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H

InChI key

ZMMJGEGLRURXTF-UHFFFAOYSA-N

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This Item
E1510E7637E8751
Ethidium bromide solution BioReagent, for molecular biology, 500 μg/mL in H2O

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Ethidium bromide BioReagent, for molecular biology, powder

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E7637

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Essential+ Grade
Ethidium bromide ~95% (HPLC)

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E8751

Ethidium bromide

-
product line

BioReagent

product line

BioReagent

product line

BioReagent

product line

-

concentration

500 μg/mL in H2O

concentration

10 mg/mL in H2O

concentration

-

concentration

-

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

-

suitability

suitable for gel electrophoresis

suitability

suitable for gel electrophoresis

suitability

suitable for gel electrophoresis

suitability

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Application

Ethidium bromide solution has been used to deplete mtDNA to investigate mitochondrial damage signals in autophagy. It has also been used to detect DNA damage using comet assay.
Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.

Biochem/physiol Actions

Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

Reconstitution

For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Ethidium bromide is a well-known and widely used fluorescent dye in biotechnology research.

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