E1388

Sigma-Aldrich

β-Endothelial Cell Growth Factor human

β-ECGF, recombinant, expressed in E. coli, suitable for cell culture

Synonym(s):
ECGS, β-ECGF
CAS Number:
MDL number:
NACRES:
NA.77
Pricing and availability is not currently available.

biological source

human

recombinant

expressed in E. coli

assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

0.03-0.3 ng/mL ED50/EC50

quality

endotoxin tested

mol wt

predicted mol wt ~17 kDa

packaging

pkg of 25 μg

storage condition

avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)

application(s)

cell culture | mammalian: suitable

impurities

≤1.0 EU/μg protein Endotoxin level (LAL test)

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... VEGFB(7423)

General description

The β-endothelial cell growth factor (b-ECGF) gene encodes an angiogenic factor, and is mapped to human chromosome 11q13.1. Recombinant, human b-endothelial cell growth factor (b-ECGF) is a 14 amino acid N-terminal extension of acidic fibroblast growth factor. It is expressed in Escherichia coli. The 154 amino acid recombinant protein, generated after removal of the initiation methionine, has a predicted molecular mass of about 17 kDa.

Application

β-Endothelial Cell Growth Factor human has been used:
  • in cell death assay
  • in endothelial cell culture
  • in human umbilical vascular endothelial cell (HUVEC) culture

Biochem/physiol Actions

β-Endothelial cell growth factor (β-ECGF) is a potent endothelial cell mitogen and its mitogenic activity is potentiated by heparin. β-ECGF stimulates the growth of quiescent HUVEC cells in culture. β-ECGF is associated with lipid and glucose metabolism. This growth factor induces neovascularization in disease conditions. Upregulation of β-ECGF is observed in diabetic retinopathy, and polycystic ovary syndrome. It is also associated with tumor proliferation and invasion. β-ECGF increases fatty acid transport proteins expression to improve endothelial mediated lipid transpor.

Other Notes

14 amino acid, N-terminal extended form of human FGF-acidic.

Physical form

Lyophilized from a 0.2 μm filtered solution in 10 mM MOPS, 50 mM ammonium sulfate, 0.2 mM DTT, and 0.2 mM EDTA, pH 7.2, containing 50 μg bovine serum albumin per 1 μg cytokine.

Analysis Note

The proliferative activity is tested in culture by using quiescent NR6R-3T3 fibroblasts.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Apoptosis-inducing-factor-dependent mitochondrial function is required for T cell but not B cell function
Milasta S, et al.
Immunity, 44(1), 88-102 (2016)
Endothelial cells provide a niche for placental hematopoietic stem/progenitor cell expansion through broad transcriptomic modification
Raynaud CM, et al.
Stem Cell Research, 11(3), 1074-1090 (2013)
Vascular endothelial growth factor B and its signaling
Lal N, et al.
Frontiers in cardiovascular medicine, 5(18), 39-39 (2018)
Bioinformatics analyses of the role of vascular endothelial growth factor in patients with non-small cell lung cancer
Wang Y, et al.
PLoS ONE, 10(9), e0139285-e0139285 (2015)
A blood pressure-associated variant of the SLC39A8 gene influences cellular cadmium accumulation and toxicity
Zhang R, et al.
Human Molecular Genetics, 25(18), 4117-4126 (2016)

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