E1894

EPPS

BioXtra, ≥99.5% (titration)

• Synonym(s): Triethanolamine hydrochloric acid salt, 4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic acid, 4-(2-Hydroxyethyl)piperazine-1-propanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(3-propanesulfonic acid), HEPPS
• Empirical Formula (Hill Notation): C₉H₂₀N₂O₄S
• CAS Number: 16052-06-5
• Molecular Weight: 252.33
• Beilstein/REAXYS Number: 3957385
• EC Number: 240-198-8
• MDL number: MFCD00006160
• UNSPSC Code: 12161700
• PubChem Substance ID: 24894443
• NACRES: NA.25

Properties

• product line: BioXtra
• Quality Level: 400
• assay: ≥99.5% (titration)
• form: powder
• impurities: ≤0.005% Phosphorus (P); ≤0.1% Insoluble matter
• ign. residue: ≤0.1%
• pH: 5.0-7.0 (20 °C, 1 M in H₂O)
• useful pH range: 7.3-8.7
• pKa: 8
• mp: 237-239 °C (lit.)
• solubility: H₂O: 1 M at 20 °C, clear, colorless
• anion traces: bromide (Br^-): ≤0.1%
• cation traces: Al: ≤0.0005%; Ca: ≤0.002%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Mg: ≤0.0005%; NH₄⁺: ≤0.1%; Na: ≤0.005%; Pb: ≤0.001%; Zn: ≤0.0005%
• absorption: ≤0.1 at 260 in H₂O at 1 M; ≤0.1 at 280 in H₂O at 1 M
• application(s): diagnostic assay manufacturing
• SMILES string: OCCN1CCN(CCCS(O)(=O)=O)CC1
• InChI: 1S/C9H20N2O4S/c12-8-7-11-5-3-10(4-6-11)2-1-9-16(13,14)15/h12H,1-9H2,(H,13,14,15)
• InChI key: OWXMKDGYPWMGEB-UHFFFAOYSA-N

Description

General description

EPPS, also known as 4-(2-hydroxyethyl)piperazine-1-propanesulfonic acid, is a zwitterionic buffering agent that belongs to the group of good buffers.[1] It plays a vital role in maintaining a stable pH in solutions, which is critical for various biological and biochemical processes. EPPS buffer finds extensive application in biological research and cell culture experiments.[2]

Application

EPPS buffer has been used as a component of buffer solution for methylglyoxal (MG) treatment in vitro to form advanced glycation end products (AGEs) to crosslink collagen fibrils.[3][4] It has also been used in the preparation of fixation buffer solution for light microscopy.[5]

Features and Benefits

• The BioXtra product line ensures T15 high-quality products for consistent results.
• A high assay of ≥99.5% guarantees the purity of the product.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)