E2264

Endoglycosidase F3 from Elizabethkingia miricola

recombinant, expressed in E. coli, 30 U/mg

• Synonym(s): Elizabethkingia miricola, Endo-β-N-acetylglucosaminidase F3, Endoglycosidase F3 from Elizabethkingia (Chryseobacterium/Flavobacterium) meningosepticum
• Enzyme Commission number: 3.2.1.96 (BRENDA, IUBMB)
• MDL number: MFCD00146843
• NACRES: NA.32

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• conjugate: (N-linked)
• form: solution
• specific activity: 30 U/mg
• mol wt: 32 kDa
• shipped in: wet ice
• storage temp.: 2-8°C

Description

Application

Endoglycosidase F3 from Elizabethkingia miricola has been used to analyze core fucosylation and tryptic digests of serum proteins.

Packaging

0.1, 0.2, 0.3 unit

Supplied with 5× Reaction Buffer, 250 mM sodium acetate, pH 4.5

Biochem/physiol Actions

Endoglycosidase F3 belongs to the glycoside hydrolase family 18 (GH18). It has hydrolytic activity. Endoglycosidase F3 glycosylates α-1,6-fucosylated GlcNAc derivative to give natural, core fucosylated complex-type N-glycopeptides.

Cleaves asparagine-linked biantennary and triantennary complex, oligosaccharides depending on the state of core fucosylation and peptide linkage.

Unit Definition

One unit will release N-linked oligosaccharides from 1 μmole of denatured porcine fibrinogen in 1 minute at 37 °C, pH 4.5.

Physical form

Aseptically filled solution in 20 mM Tris-HCl, pH 7.5

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves