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MilliporeSigma

E2636

Sigma-Aldrich

ExtrAvidin® –Alkaline Phosphatase

buffered aqueous solution

Synonym(s):

Alkaline Phosphatase Reagent, ExtrAvidin Alkaline Phosphatase

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$118.00
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$238.00
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$903.00

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0.2 ML
$118.00
0.5 ML
$238.00
5 X 0.5 ML
$903.00

About This Item

MDL number:
MFCD00163600
UNSPSC Code:
12352203
NACRES:
NA.46

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$118.00

In StockDetails

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conjugate

alkaline phosphatase conjugate

Quality Level

200

form

buffered aqueous solution

technique(s)

dot blot: 1:300,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues sections
indirect ELISA: 1:60,000

shipped in

ambient

storage temp.

2-8°C

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This Item
E2761E2886A7294
ExtrAvidin® –Alkaline Phosphatase buffered aqueous solution

E2636

ExtrAvidin® –Alkaline Phosphatase

ExtrAvidin®−FITC buffered aqueous solution

E2761

ExtrAvidin®−FITC

ExtrAvidin®−Peroxidase buffered aqueous solution

E2886

ExtrAvidin®−Peroxidase

Avidin–Alkaline Phosphatase buffered aqueous solution

A7294

Avidin–Alkaline Phosphatase

technique(s)

dot blot: 1:300,000, indirect ELISA: 1:60,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues sections

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20 μg/mL using human tissues, indirect immunofluorescence: 1:200

technique(s)

direct ELISA: 1:2,000, dot blot: 1:4,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

technique(s)

direct ELISA: 1:70,000, western blot: 1:150,000-1:300,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per lane

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

conjugate

alkaline phosphatase conjugate

conjugate

FITC conjugate

conjugate

peroxidase conjugate

conjugate

alkaline phosphatase conjugate

General description

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs.[1] It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii.[2] ExtrAvidin has been conjugated to Alkaline Phosphatase and is used for various techniques.

Application

ExtrAvidin® -Alkaline Phosphatase has been used in:
  • Dot blot
  • Immunohistochemistry (formalin-fixed, paraffin-embedded sections)
  • Indirect ELISA
  • Enzyme linked immunosorbent assay (ELISA)[3][4]
  • Enzyme-linked immunosorbent spot (ELISPOT) analysis[5]
  • Competitive ELISA[6]

Biochem/physiol Actions

Alkaline Phosphatase catalyzes the hydrolysis of esters to yield phosphoric acid. It is also facilitate the transport of substances across membranes.[7]

Physical form

Solution in 0.05 M Tris-HCl buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin and 15 mM sodium azide

Preparation Note

Affinity purified protein

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000245279
0000269020
0000294112
0000245279
0000294112

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