Unfortunately, information regarding the composition of this product is not available. Endothelial cell growth supplement (ECGS) is an extract of bovine neural tissue containing growth-promoting factors for vascular endothelial cells of mammalian origin. The composition of the products has not been analyzed. According to literature, ECGS comprises acidic fibroblast growth factor (aFGF) and basic fibroblast growth factor (bFGF).
E2759
Endothelial cell growth supplement from bovine neural tissue
powder, suitable for cell culture
Synonym(s):
ECGS, Endothelial Mitogen
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Product Name
Endothelial cell growth supplement from bovine neural tissue, ECGS, suitable for cell culture
biological source
bovine neuroblastoma
form
powder
potency
2-200 μg/mL ED50/EC50
quality
endotoxin tested
packaging
pkg of 15 and 5X15 mg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤20 EU/vial (LAL test)
color
tan to brown
solubility
water: soluble (1 vial reconstituted with 1 ml 0.85% NaCl)
storage temp.
2-8°C
Quality Level
1 of 4
This Item | E0760 | E4127 | F3133 |
|---|---|---|---|
| biological source bovine neuroblastoma | biological source bovine pituitary glands | biological source mouse submaxillary glands | biological source bovine pituitary glands |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form powder | form powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| impurities ≤20 EU/vial (LAL test) | impurities ≤20 EU/vial (LAL test) | impurities ≤50 EU/vial (LAL test) | impurities endotoxin, tested |
Analysis Note
The proliferative activity of endothelial cell growth supplement (ECGS) is tested in culture using fetal bovine heart endothelial cells (ATCC CRL 1395) seeded at low density. The EC50 is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay.
Application
Biochem/physiol Actions
Endothelial cell growth supplement (ECGS) improves proliferation of human saphenous vein endothelial cells (HSVEC) and human umbilical vein endothelial cells (HUVEC).[4] It coordinates with interleukin 1 (IL-1) and mediates matrix remodelling in vascular smooth muscle cells (VSMCs).[5] It is essential for endothelial cells proliferation and adipose-derived stem cell (ASC) differentiation.[6] An extract of bovine neural tissue containing growth promoting factors for vascular endothelial cells of mammalian origin. ECGS has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production.[7]
General description
Endothelial cell growth supplement (ECGS) comprises acidic fibroblast growth factor (aFGF) and basic FGF (bFGF).[5]
Preparation Note
ECGS is an extract of bovine neural tissue containing growth promoting factors for vascular endothelial cells of mammalian origin. Endothelial cell growth supplement is prepared using a modification of the method of Maciag, et al., and is lyophilized from a sterile solution containing NaCl and streptomycin sulfate.
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Human macrovascular endothelial cells: optimization of culture conditions
Terramani TT, et al.
In Vitro Cellular & Developmental Biology. Animal, 36(2), 125-132 (2000)
Endothelial differentiation of adipose-derived stem cells: effects of endothelial cell growth supplement and shear force
Fischer LJ, et al.
The Journal of Surgical Research, 152(1), 157-166 (2009)
Soluble adhesion molecules correlate with surface expression in an in vitro model of endothelial activation
Kjaergaard AG, et al.
Basic and Clinical Pharmacology and Toxicology, 113(4), 273-279 (2013)
KML001 displays vascular disrupting properties and irinotecan combined antitumor activities in a murine tumor model
Moon CH, et al.
Testing, 8(1), e53900-e53900 (2013)
Heparin/endothelial cell growth supplement regulates matrix gene expression and prolongs life span of vascular smooth muscle cells through modulation of interleukin-1
Hsu JY, et al.
In Vitro Cellular & Developmental Biology. Animal, 35(10), 647-654 (1999)
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Instructions
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Apart from FGF, is there any other growth factor in the supplement (eg. VEGF)?
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