Esterase from porcine liver

ammonium sulfate suspension, ≥150 units/mg protein (biuret)

PLE, Carboxylic-ester hydrolase, Carboxyl esterase
CAS Number:
Enzyme Commission number:
MDL number:

Quality Level


ammonium sulfate suspension

specific activity

≥150 units/mg protein (biuret)

mol wt

168 kDa

storage temp.


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General description

Esterase, also known as carboxyl ester hydrolase is a member of the hydrolase superfamily of enzymes.


Esterase from porcine liver has been used to unmask the fluorescence in ultraviolet-visible spectroscopy.
The enzyme from Sigma has been used to develop an alternative gel-based protocol for screening for bead-bound catalytic activity in aqueous media. The product has also been used to investigate the properties of the compound L-valine-3-{8-[(E)-2-[3-methoxyphenyl)ethenyl]-7-methyl-1-propargylxanthine-3-yl}propyl ester hydrochloride (MSX-4, an aminoacid ester prodrug of the adenosine A2A receptor antagonist MSX-2).
Porcine liver esterase is used to catalyze the hydrolysis of pentaacetyl catechin and epicatechin for use in pharmaceutical and industrial applications.

Pig liver esterase is commonly used for kinetic resolutions and assymetric synthesis in organic chemistry.

Biochem/physiol Actions

Esterase enzyme of bacteria and fungi seems to have several industrial applications. It is capable of catalyzing the hydrolysis and synthesis of ester bonds.
Pig liver esterase catalyzes enantioselective conversion of an ester to a carboxylic acid. The molecular weight is found to be 168 kDa. It is a serine enzyme with two active sites on each molecule, which dissociates into active half-molecules in the presence of dilute acid or concentrated salts.
Esterase acts on water-soluble carboxyl esters containing short chain fatty acids. Its functionality is attributed to the catalytic triad of Ser, His and Asp/Glu.

Unit Definition

One unit will hydrolyze 1.0 μmole of ethyl butyrate to butyric acid and ethanol per min at pH 8.0 at 25 °C.

Physical form

Suspension in 3.2 M (NH4)2SO4, pH 8

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Karl Vollmann et al.
Molecules (Basel, Switzerland), 13(2), 348-359 (2008-02-29)
The compound L-valine-3-{8-[(E)-2-[3-methoxyphenyl)ethenyl]-7-methyl-1-propargylxanthine-3-yl}propyl ester hydrochloride (MSX-4) was synthesized as an amino acid ester prodrug of the adenosine A2A receptor antagonist MSX-2. It was found to be stable in artificial gastric acid, but readily cleaved by pig liver esterase.
Fluorogenic affinity label for the facile, rapid imaging of proteins in live cells
Watkins RW, et al.
Organic & Biomolecular Chemistry, 7(19), 3969-3975 (2019)
Robert L Wilensky et al.
Nature medicine, 14(10), 1059-1066 (2008-09-23)
Increased lipoprotein-associated phospholipase A(2) (Lp-PLA(2)) activity is associated with increased risk of cardiac events, but it is not known whether Lp-PLA(2) is a causative agent. Here we show that selective inhibition of Lp-PLA(2) with darapladib reduced development of advanced coronary...
F Dorandeu et al.
Toxicology, 248(2-3), 151-157 (2008-05-03)
Organophosphorus chemical warfare agents (nerve agents) are to be feared in military operations as well as in terrorist attacks. Among them, VX (O-ethyl-S-[2-(diisopropylamino)ethyl] methylphosphonothioate) is a low volatility liquid that represents a percutaneous as well as an inhalation hazard if...
Pig liver esterase. Reactions with alcohols, structure-reactivity correlations, and the acyl-enzyme intermediate.
P Greenzaid et al.
Biochemistry, 10(7), 1210-1222 (1971-03-30)
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Read More
Objective: To standardize a procedure for the enzymatic determination of Esterase activity using Ethyl Butyrate as a substrate.
Read More

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