Yes, casein or nonfat dry milk may be used as a blocking solution. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/370/767/e2886dat-ms.pdf
E2886
ExtrAvidin®−Peroxidase
buffered aqueous solution
Synonym(s):
ExtrAvidin HRP
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About This Item
Recommended Products
conjugate
peroxidase conjugate
form
buffered aqueous solution
technique(s)
direct ELISA: 1:2,000
dot blot: 1:4,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
shipped in
wet ice
storage temp.
2-8°C
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This Item | E2636 | E4142 | SAB2702288 |
---|---|---|---|
technique(s) direct ELISA: 1:2,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, dot blot: 1:4,000 | technique(s) dot blot: 1:300,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues sections, indirect ELISA: 1:60,000 | technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 | technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:1,000, western blot: 1:5,000-1:20,000 |
form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution |
shipped in wet ice | shipped in ambient | shipped in wet ice | shipped in wet ice |
storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
conjugate peroxidase conjugate | conjugate alkaline phosphatase conjugate | conjugate CY3 conjugate | conjugate unconjugated |
Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
Specificity
Application
Biochem/physiol Actions
Physical form
Preparation Note
Storage and Stability
Legal Information
Disclaimer
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Biotinylated protein을 detection하기 위하여 ExtrAvidin-peroxidase를 ELISA에 사용하고자하는데, well blocking solution으로 casein이나 Nonfat Dry Milk 등을 사용해도 괜찮을까요?
1 answer-
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