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E2886

Sigma-Aldrich

ExtrAvidin®−Peroxidase

buffered aqueous solution

Synonym(s):

ExtrAvidin HRP

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0.2 ML
$127.00
1 ML
$409.00
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$1,650.00

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0.2 ML
$127.00
1 ML
$409.00
5 X 1 ML
$1,650.00

About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

$127.00


In StockDetails


Request a Bulk Order

conjugate

peroxidase conjugate

form

buffered aqueous solution

technique(s)

direct ELISA: 1:2,000
dot blot: 1:4,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

shipped in

wet ice

storage temp.

2-8°C

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1 of 4

This Item
E2636E4142SAB2702288
technique(s)

direct ELISA: 1:2,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, dot blot: 1:4,000

technique(s)

dot blot: 1:300,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues sections, indirect ELISA: 1:60,000

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:1,000, western blot: 1:5,000-1:20,000

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

shipped in

wet ice

shipped in

ambient

shipped in

wet ice

shipped in

wet ice

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

conjugate

peroxidase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

CY3 conjugate

conjugate

unconjugated

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

Specificity

The product is found to be reactive with Anti-Avidin, Catalog Number A5170, and non-reactive with Anti-Streptavidin, Catalog Number S6390, by immunoelectrophoresis.

Application

ExtrAvidin®-Peroxidase is suitable for the following applications:
  • Dot blot at a minimum dilution of 1:4,000
  • Immunohistochemistry (using formalin-fixed, paraffin-embedded sections at a dilution of 1:100)[1][2][3][4][5][6]
  • Direct ELISA at a minimum dilution of 1:2,000[7][8]
It has also been used for:
  • Immunoprecipitation and Immunobloting[9]
  • Ra2I solid-phase binding assays[10]
  • Southwestern blotting[11]
  • Western blotting[11][12][13]
  • Immunocytochemistry[14]

Biochem/physiol Actions

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs.[15] It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii.[16] ExtrAvidin-Peroxidase may be used in conjunction with biotin in different techniques.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Preparation Note

Affinity purified protein

Storage and Stability

Store at 2-8 °C. Do Not Freeze. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Questions

  1. Biotinylated protein을 detection하기 위하여 ExtrAvidin-peroxidase를 ELISA에 사용하고자하는데, well blocking solution으로 casein이나 Nonfat Dry Milk 등을 사용해도 괜찮을까요?

    1 answer
    1. Yes, casein or nonfat dry milk may be used as a blocking solution. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/370/767/e2886dat-ms.pdf

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