E2886

ExtrAvidin^®−Peroxidase

buffered aqueous solution

• Synonym(s): ExtrAvidin HRP
• MDL number: MFCD00163600
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• conjugate: peroxidase conjugate
• form: buffered aqueous solution
• technique(s): direct ELISA: 1:2,000; dot blot: 1:4,000; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
• shipped in: wet ice
• storage temp.: 2-8°C

Description

Specificity

The product is found to be reactive with Anti-Avidin, Catalog Number A5170, and non-reactive with Anti-Streptavidin, Catalog Number S6390, by immunoelectrophoresis.

Application

ExtrAvidin^®-Peroxidase is suitable for the following applications:

• Dot blot at a minimum dilution of 1:4,000
• Immunohistochemistry (using formalin-fixed, paraffin-embedded sections at a dilution of 1:100)[1][2][3][4][5][6]
• Direct ELISA at a minimum dilution of 1:2,000[7][8]

It has also been used for:

• Immunoprecipitation and Immunobloting[9]
• Ra2I solid-phase binding assays[10]
• Southwestern blotting[11]
• Western blotting[11][12][13]
• Immunocytochemistry[14]

Biochem/physiol Actions

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs.[15] It consists of four high affinity binding sites for biotin. ExtrAvidin^® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii.[16] ExtrAvidin-Peroxidase may be used in conjunction with biotin in different techniques.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Preparation Note

Affinity purified protein

Storage and Stability

Store at 2-8 °C. Do Not Freeze. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)