E3004

Extract-N-Amp^™ PCR ReadyMix^™

Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

• Synonym(s): Plant direct PCR master mix, Tissue direct PCR master mix
• NACRES: NA.55

Properties

• Quality Level: 200
• usage: sufficient for 100 reactions; 1.2 mL sufficient for 100 amplifications; sufficient for 1000 reactions; 12 mL sufficient for 1000 amplifications; sufficient for 10000 reactions; 125 mL sufficient for 10000 amplifications
• feature: dNTPs included; hotstart
• color: colorless
• application(s): agriculture
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Extract-N-Amp^™ PCR ReadyMix^™ is a specially formulated hot start PCR master mix for direct PCR amplification from the extract.

PCR ReadyMix is intended for use with Sigma′s Extract-N-Amp Plant PCR kit and Extract-N-Amp Tissue PCR Kit. All Extract-N-Amp kits include a PCR ReadyMix sufficient for one PCR reaction per extraction. However, if additional PCR reactions are required, supplemental PCR ReadyMix may be needed.

Application

Extract-N-Amp^™ PCR ReadyMix^™ has been used for the following applications:

• Genomic DNA extraction
• Fungal DNA extraction and amplification by polymerase chain reaction (PCR) reactions
• Direct PCR amplification
• Genotyping
• Real-Time PCR

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable