The amplified DNA should be loaded onto an agarose gel after the PCR is completed by adding a separate loading buffer or tracking dye, such as Gel Loading Solution, Catalog Number G2526.
E3004
Extract-N-Amp™ PCR ReadyMix™
Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue
Extract-N-Amp™ PCR ReadyMix™
Synonym(s):
Plant direct PCR master mix, Tissue direct PCR master mix
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Select a Size
1.2 ML
$180.00
12 ML
$1,780.00
125 ML
$12,180.00
About This Item
NACRES:
NA.55
UNSPSC Code:
41106303
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Product Name
Extract-N-Amp™ PCR ReadyMix™, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue
usage
sufficient for 100 reactions
1.2 mL sufficient for 100 amplifications
sufficient for 1000 reactions
12 mL sufficient for 1000 amplifications
sufficient for 10000 reactions
125 mL sufficient for 10000 amplifications
feature
dNTPs included
hotstart
color
colorless
application(s)
agriculture
shipped in
wet ice
storage temp.
−20°C
Quality Level
1 of 4
This Item | R4775 | R2523 | P0982 |
|---|---|---|---|
| usage sufficient for 100 reactions | usage sufficient for 100 reactions, 1.2 mL sufficient for 100 amplifications, sufficient for 1000 reactions, 12 mL sufficient for 1000 amplifications, sufficient for 10000 reactions, 125 mL sufficient for 10000 amplifications | usage sufficient for 100 reactions, sufficient for 20 reactions | usage sufficient for 100 reactions, sufficient for 20 reactions, sufficient for 800 reactions |
| feature dNTPs included | feature dNTPs included, hotstart | feature dNTPs included, hotstart: no | feature dNTPs included, hotstart |
| color colorless | color red | color red | color red |
microbialanalysis | - | - | - |
| application(s) agriculture | application(s) agriculture | application(s) agriculture | application(s) agriculture |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
Application
General description
Extract-N-Amp™ PCR ReadyMix™ is a specially formulated hot start PCR master mix for direct PCR amplification from the extract.
PCR ReadyMix is intended for use with Sigma′s Extract-N-Amp Plant PCR kit and Extract-N-Amp Tissue PCR Kit. All Extract-N-Amp kits include a PCR ReadyMix sufficient for one PCR reaction per extraction. However, if additional PCR reactions are required, supplemental PCR ReadyMix may be needed.
Legal Information
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
related product
Product No.
Description
Pricing
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Muhammad Iqbal et al.
Genome, 50(5), 511-516 (2007-07-07)
Vernalization response (Vrn) genes play a major role in determining the flowering/maturity times of spring-sown wheat. We characterized a representative set of 40 western Canadian adapted spring wheat cultivars/lines for 3 Vrn loci. The 40 genotypes were screened, along with
A Kamran et al.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 126(8), 1965-1976 (2013-05-08)
Earliness per se regulates flowering time independent of environmental signals and helps to fine tune the time of flowering and maturity. In this study, we aimed to map earliness per se quantitative trait loci (QTLs) affecting days to flowering and
Anneke van der Zee et al.
PloS one, 11(3), e0150755-e0150755 (2016-03-10)
The objective of our study was the development of a semi-quantitative real-time PCR to detect uropathogens. Two multiplex PCR reactions were designed to detect Escherichia coli, Klebsiella spp., Enterobacter spp., Citrobacter spp., Proteus mirabilis, Enterococcus faecalis, and Pseudomonas aeruginosa. 16S
Melinda Belisle et al.
Microbial ecology, 63(4), 711-718 (2011-11-15)
Microfungi that inhabit floral nectar offer unique opportunities for the study of microbial distribution and the role that dispersal limitation may play in generating distribution patterns. Flowers are well-replicated habitat islands, among which the microbes disperse via pollinators. This metapopulation
Huirong Gao et al.
Frontiers in plant science, 11, 535-535 (2020-05-21)
Modern maize hybrids often contain biotech and native traits. To-date all biotech traits have been randomly inserted in the genome. Consequently, developing hybrids with multiple traits is expensive, time-consuming, and complex. Here we report using CRISPR-Cas9 to generate a complex
Articles
Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.
Extract-N-Amp™ Tissue PCR Kit quickly extracts PCR-ready DNA from mouse tails, providing optimized PCR mix for efficient amplification.
Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.
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Sehr geehrte Damen und Herren, sollen wir nach dem PCR Lauf, Ladepuffer dazugeben?
1 answer-
Helpful?
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Can I use the TriTrack DNA Loading Dye or the TrakitCyanoOrange Loading Buffer with the ReadyMix??
1 answer-
The choice of loading dye will not impact the performance the the E3004 - Extract-N-Amp™ PCR ReadyMix. While we have not tested loading dyes outside of the product, G2526, any commercial DNA loading dye is expected to be compatible.
Helpful?
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What is the polymerase used for this kit? I am trying to calculate Tm for my PCR, but I would need to know what type of polymerase this kit uses. I am guessing something close to Q5 Hotstart?
1 answer-
This kit employs Taq DNA polymerase (Product D6677).
Helpful?
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