E3138

Sigma-Aldrich

Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse

clone F4, ascites fluid

Synonym(s):
Anti-EGFR
MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

F4, monoclonal

contains

15 mM sodium azide

species reactivity

human

packaging

antibody small pack of 25 μL

application(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:2,000

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... EGFR(1956)

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General description

Monoclonal Anti-Epidermal Growth Factor (EGF) Receptor (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The receptor for epidermal growth factor is an integral cell membrane protein of 170 kD, which spans the membranes of a wide range of normal and malignant epithelial cells. EGF-R have been identified in sarcomas, gliomas, breast, bladder, lung and gynecological tumors. The EGF-R has a half-life of approximately 10 hours in human fibroblasts, but in the presence of EGF this value is reduced to about 1 hour.

Specificity

This antibody recognizes specifically an intracellular domain of the EGF receptor.

Immunogen

synthetically produced residues 985-996 of the human EGF receptor.

Application

Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse has been used in:
  • western blotting
  • immunoprecipitation
  • immunocytochemistry
  • immunohistochemical staining

Biochem/physiol Actions

EGFR is a transmembrane glycoprotein that binds to the EGF family. It is a pivotal regulator of cell proliferation, growth, differentiation and DNA repair after radiation-induced damage. Mutations in the gene encoding EGFR are associated with a number of malignancies including lung cancer and colorectal cancer.
Epidermal growth factor receptor (EGF-R) is a tyrosine-specific protein kinase with the capacity to phosphorylate tyrosine residues located near its carboxy-terminus.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Localization of the epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) in the bovine testis
Kassab M, et al.
Journal of Molecular Histology, 38(3), 207-214 (2007)
Changes in expression of epidermal growth factor receptors by anterior pituitary cells during the estrous cycle: cyclic expression by gonadotropes
Armstrong J and Childs GV
Endocrinology, 138(5), 1903-1908 (1997)
T Cerny et al.
British journal of cancer, 54(2), 265-269 (1986-08-01)
Epidermal growth factor receptor (EGF-R) expression was assessed in 63 lung tumour samples with a monoclonal antibody (EGF-R1) by indirect immunoperoxidase staining on cryostat sections. All 15 small cell lung cancer samples were negative whereas over 80% of the 48...
Hyaluronan-induced masking of ErbB2 and CD44-enhanced trastuzumab internalisation in trastuzumab resistant breast cancer
Palyi-KZ, et al.
European Journal of Cancer, 43(16), 2423-2433 (2007)
Tyrosine phosphorylation mapping of the epidermal growth factor receptor signaling pathway
Steen H, et al.
The Journal of Biological Chemistry, 277(2), 1031-1039 (2002)

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