If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf
E3889
Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid
Molecular Biology, ≥97.0%
Synonym(s):
EGTA, Egtazic acid, Ethylene-bis(oxyethylenenitrilo)tetraacetic acid, Glycol ether diamine tetraacetic acid
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About This Item
Recommended Products
grade
Molecular Biology
assay
≥97.0%
form
powder
reaction suitability
reagent type: chelator
loss
≤1.0% loss on drying
mp
241 °C (dec.) (lit.)
foreign activity
DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected
SMILES string
OC(=O)CN(CCOCCOCCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C14H24N2O10/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24)
InChI key
DEFVIWRASFVYLL-UHFFFAOYSA-N
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Related Categories
1 of 4
This Item | 03777 | 4100-OP | 324626 |
|---|---|---|---|
| grade for molecular biology | grade for molecular biology | grade - | grade Molecular Biology |
| assay ≥97.0% | assay ≥99.0% (T) | assay - | assay ≥98% dry basis (complexometric) |
| Quality Level 400 | Quality Level 300 | Quality Level 100 | Quality Level 100 |
| form powder | form powder | form crystalline powder (Fine) | form solid (fine) |
| mp 241 °C (dec.) (lit.) | mp 241 °C (dec.) (lit.) | mp - | mp - |
| reaction suitability reagent type: chelator | reaction suitability reagent type: chelator | reaction suitability reagent type: chelator | reaction suitability reagent type: chelator |
Application
- in Dulbecco′s phosphate-buffered saline (DPBS) to induce the weakening of cell-to-cell junctions
- as an extracellular calcium chelator to study the role of calcium in axonal mitochondria degeneration
- as a component in lysis buffer for lysing mouse mucosal cells for western blot analysis
Biochem/physiol Actions
Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
ppe
Eyeshields, Gloves, type N95 (US)
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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