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E3889

Sigma-Aldrich

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid

Molecular Biology, ≥97.0%

Synonym(s):

EGTA, Egtazic acid, Ethylene-bis(oxyethylenenitrilo)tetraacetic acid, Glycol ether diamine tetraacetic acid

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10 G
$105.00
25 G
$184.00
100 G
$497.00
500 G
$1,790.00

About This Item

Linear Formula:
[-CH2OCH2CH2N(CH2CO2H)2]2
CAS Number:
Molecular Weight:
380.35
Beilstein/REAXYS Number:
1717370
EC Number:
MDL number:
UNSPSC Code:
12352106
PubChem Substance ID:
NACRES:
NA.31

$105.00


In StockDetails


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grade

Molecular Biology

assay

≥97.0%

form

powder

reaction suitability

reagent type: chelator

loss

≤1.0% loss on drying

mp

241 °C (dec.) (lit.)

foreign activity

DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected

SMILES string

OC(=O)CN(CCOCCOCCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C14H24N2O10/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24)

InChI key

DEFVIWRASFVYLL-UHFFFAOYSA-N

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1 of 4

This Item
037774100-OP324626
grade

for molecular biology

grade

for molecular biology

grade

-

grade

Molecular Biology

assay

≥97.0%

assay

≥99.0% (T)

assay

-

assay

≥98% dry basis (complexometric)

Quality Level

400

Quality Level

300

Quality Level

100

Quality Level

100

form

powder

form

powder

form

crystalline powder (Fine)

form

solid (fine)

mp

241 °C (dec.) (lit.)

mp

241 °C (dec.) (lit.)

mp

-

mp

-

reaction suitability

reagent type: chelator

reaction suitability

reagent type: chelator

reaction suitability

reagent type: chelator

reaction suitability

reagent type: chelator

Application

A chelating agent useful for the determination of calcium in the presence of magnesium.
Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid has been used:
  • in Dulbecco′s phosphate-buffered saline (DPBS) to induce the weakening of cell-to-cell junctions
  • as an extracellular calcium chelator to study the role of calcium in axonal mitochondria degeneration
  • as a component in lysis buffer for lysing mouse mucosal cells for western blot analysis

Biochem/physiol Actions

Ethylene glycol tetraacetic acid (EGTA) can chelate calcium (Ca2+) at a ratio of 1:1. It serves as an anti-coagulant when dissolved at 1 g per 100 ml of blood. EGTA can prevent the vesicular release in the nanodomain of single Ca2+ channels. This metal chelator can inhibit the activity of pseudolysin.
Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid A is a chelating agent used for the determination of calcium in the presence of magnesium.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

ppe

Eyeshields, Gloves, type N95 (US)


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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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