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E4013

Sigma-Aldrich

Monoclonal Anti-Elastin antibody produced in mouse

clone BA-4, ascites fluid

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Synonym(s):
Anti-ADCL1, Anti-SVAS, Anti-WBS, Anti-WS
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

BA-4, monoclonal

mol wt

antigen 67.5 kDa

contains

15 mM sodium azide

species reactivity

human, goat, sheep, guinea pig, pig, canine, feline, bovine

packaging

antibody small pack of 25 μL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:5,000 using sheep arterial tissue
microarray: suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ELN(2006)

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M7786M8421P9318
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

clone

BA-4, monoclonal

clone

hSM-V, monoclonal

clone

NOQ7.5.4D, monoclonal

clone

7A8, monoclonal

species reactivity

human, goat, sheep, guinea pig, pig, canine, feline, bovine

species reactivity

guinea pig, human, pig, canine, rabbit, chicken

species reactivity

sheep, rat, bovine, hamster, pig, canine, feline, goat, chicken, mouse, rabbit, human, guinea pig

species reactivity

marsupial (Ptoorous tridactylis PtK2 cell line), rat

Gene Information

human ... ELN(2006)

Gene Information

human ... MYH11(4629)

Gene Information

human ... MYH7(4625)
mouse ... Myh7(140781)
rat ... Myh7(29557)

Gene Information

rat ... Plec1(64204)

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

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General description

Monoclonal Anti-Elastin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Elastin is an important polymeric protein of connective tissue. It is synthesized and secreted as a soluble single-chain protein (tropoelastin) that undergoes numerous post-ribosomal modifications prior to the organization of the elastic fiber in the extracellular space.

Specificity

The antibody binds to insoluble elastin, α-elastin, tropoelastin (the soluble non-cross linked precursor of elastin) and to peptide fragments generated by proteolytic digestion of insoluble elastin. In tropoelastin preparations, three immunoreactive bands have been observed at 67.5 kDa, 65 kDa and 62 kDa corresponding to the three elastin isoforms. The antibody is specific for an epitope composed of the repeated sequence in elastin: Val-Gly-Val-Ala-Pro-Gly. This epitope has been shown to be a chemoattractant for fibroblasts and monocytes.

Immunogen

bovine α-elastin.

Application

Monoclonal Anti-Elastin antibody produced in mouse has been used in:
  • immunohistochemistry
  • immunostaining
  • immunoblotting

Biochem/physiol Actions

Elastin is encoded by the ELN gene in humans. It is a polymeric protein in connective tissue that has exceptional properties of extension, elastic recoil to the extracellular matrix and allows many tissues in the body to resume their shape after stretching or contracting. Elastin is a self-assembling, extracellular-matrix protein providing tissue elasticity and is predominantly comprised of cross-linked tropoelastin. It plays an important role in the atherosclerotic process. The conformational disorder is caused due to a constitutive feature of elastin structure and function.
Elastin undergoes changes of morphology with aging and in many disease states. For example, abnormal accumulation of connective tissue in blood vessels contributes to alterations in vascular physiology associated with diseases such as hypertension and atherosclerosis. Several heritable diseases such as Marfan syndrome, pseudoxanthoma elasticum and the Buschke-Ollendorf syndrome may result from molecular defects of elastin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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