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E4274

Sigma-Aldrich

Endotoxin Removal solution

Remove lipopolysaccharides (LPS) from plasmid DNA preparations

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Synonym(s):
ET removal buffer, ET removal reagent, ET removal solution, endo removal buffer, endo removal reagent, endo removal solution, endotoxin contamination removal, endotoxin removal buffer, endotoxin removal reagent, plasmid purification endo, plasmid purification endo free

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E9029PLED35R2020
Sigma-Aldrich

Sigma-Aldrich

R2020

RNaseZAP

Application

Endotoxin Removal solution has been used to remove lipopolysaccharide (LPS) from Baker′s yeast RNA.

Biochem/physiol Actions

The reagent relies on the micellar properties of Triton X-114 to remove endotoxins from solution. When warmed above 20 °C, micelles aggregate into a separate phase that sequesters amphiphilic solutes, including lipopolysaccharides. The hydrophilic DNA remains in the aqueous phase.

Quantity

1 volume treats 5 volumes of DNA solution (≤1mg/ml)

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Customers Also Viewed

M Cotten et al.
Gene therapy, 1(4), 239-246 (1994-07-01)
Endotoxin (lipopolysaccharide, LPS) is commonly found as a contaminant in plasmid DNA preparations. We demonstrate here that the quantities of LPS typically contaminating DNA preparations can generate a toxicity to primary cells (primary human skin fibroblasts, primary human melanoma cells)
Determination of endotoxin through an aptamer-based impedance biosensor.
Su W
Biosensors And Bioelectronics, 32(1), 32-36 (2012)

Articles

Cell Culture FAQs: Bacterial Endotoxin Contamination

What is endotoxin? Frequently asked questions about bacterial endotoxin contamination of in vitro cell cultures. Details about how to endotoxin test using the LAL assay, common sources of laboratory endotoxin contamination and tips on how to avoid endotoxin contamination when culturing cell lines.

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