E4531

Sigma-Aldrich

Monoclonal Anti-ELKS antibody produced in mouse

clone ELKS-30, purified from hybridoma cell culture

Synonym(s):
Anti-KIAA1081
MDL number:
NACRES:
NA.41

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

ELKS-30, monoclonal

form

buffered aqueous solution

mol wt

antigen ~120 kDa

species reactivity

human, chicken, mouse, rat, bovine

concentration

~2 mg/mL

application(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.5-1 μg/mL using HeLa cell extract

isotype

IgG2a

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... ERC1(23085)
mouse ... Erc1(111173)

General description

ELKS/RAB6-interacting/CAST family member 1 is a protein encoded by the ERC1 gene in humans. It encodes for a synaptic factor and is found in the smallest region of overlap.
Monoclonal Anti-ELKS (mouse IgG2a isotype) is derived from the hybridoma ELKS-30 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human ELKS. ELKS, also known as KIAA1081, is an essential regulatory subunit of the IKK complex. It was named ELKS since 44.1% of the sequence is composed of glutamic acid (E), leucine (L), lysine (K) and serine (S) residues. The protein contains 948 amino acids with nine -helical coiled-coil domains including periodic heptad repeats that predict dimer formation. Its highest expression is in heart, placenta, lung, brain, thyroid and testis.

Application

Monoclonal Anti-ELKS antibody produced in mouse has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • fluorescence immunohistochemistry
  • immunocytochemistry
  • immunoblotting

Biochem/physiol Actions

ELKS/RAB6-interacting/CAST family member 1 is a protein encoded by the ERC1 gene in humans. It encodes for a synaptic factor and is found in the smallest region of overlap. ERC1 encodes a regulator of neurotransmitter release and acts as the best gene candidate contributing to this phenotype as well as to the autism spectrum disorder ASD. ERC1a (an isoform of ERC1) is associated with other proteins for effective migration and tumor cell invasion by stabilizing the activity at the cell front. Depletion of any of the associated protein negatively affects invasion, migration on extracellular matrix, lamellipodial persistence and the internalization of active integrin β1 receptors needed for adhesion turnover at the front of the cell.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Fusion of a novel gene, ELKS, to RET due to translocation t (10; 12)(q11; p13) in a papillary thyroid carcinoma
Nakata T, et al.
Genes Chromosomes Cancer, 25(2), 97-103 (1999)
Julien Thevenon et al.
European journal of human genetics : EJHG, 21(1), 82-88 (2012-06-21)
Speech sound disorders are heterogeneous conditions, and sporadic and familial cases have been described. However, monogenic inheritance explains only a small proportion of such disorders, in particular in cases with childhood apraxia of speech (CAS). Deletions of <5 Mb involving...
Changliang Liu et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(37), 12289-12303 (2014-09-12)
In a presynaptic nerve terminal, synaptic vesicle exocytosis is restricted to specialized sites called active zones. At these sites, neurotransmitter release is determined by the number of releasable vesicles and their probability of release. Proteins at the active zone set...
Wei Wang et al.
The Journal of biological chemistry, 290(21), 13372-13385 (2015-04-12)
DNA damage-induced NF-κB activation plays a critical role in regulating cellular response to genotoxic stress. However, the molecular mechanisms controlling the magnitude and duration of this genotoxic NF-κB signaling cascade are poorly understood. We recently demonstrated that genotoxic NF-κB activation...
Jie Chen et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 31(2), 512-525 (2011-01-14)
Synapse formation requires the organization of presynaptic active zones, the synaptic vesicle release sites, in precise apposition to postsynaptic neurotransmitter receptor clusters; however, the molecular mechanisms responsible for these processes remain unclear. Here, we show that P/Q-type and N-type voltage-dependent...

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