E4788

Sigma-Aldrich

Plant RNA/DNA Purification Kit

greener alternative

sufficient for 50 purifications

NACRES:
NA.55

Quality Level

usage

sufficient for 50 purifications

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

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General description

Norgen Biotek′s Plant RNA/DNA Purification Kit provides a rapid method for the isolation and purification of total RNA and genomic DNA simultaneously from a single sample of plants. The total RNA and genomic DNA are both column purified in under 30 minutes using a single column. It is often necessary to isolate total RNA and genomic DNA from a single plant sample, such as for studies of gene expression, mutant or transgenic plant characterization, and host plant-pathogen characterization. Traditionally the RNA and DNA would be isolated from different aliquots of a sample, however this novel technology will allow for their simultaneous isolation from the same sample. This will not only save time, but will also be of a great benefit when isolating RNA and DNA from precious, difficult to obtain or very small samples. Furthermore, gene expression analysis will be more reliable since the RNA and DNA are derived from the same sample, therefore eliminating inconsistent results.

Application

Purified RNA and DNA are of the highest quality and can be used in a number of downstream applications including:
  • Real-time PCR
  • Southern blotting
  • SNP analysis
  • Sequencing
  • AFLP
  • RFLP
  • RAPD
  • Microsatellite analysis
  • qRT-PCR
  • Primer extension
  • Expression array assays
  • Next Generation Sequencing
For simultaneous isolation of total RNA and DNA from the same plant sample.

Features and Benefits

  • Total RNA and genomic DNA are column purified simultaneously in under 30 minutes.
  • Reduces variability as RNA and DNA are isolated from a single plant sample.
  • Isolate nucleic acids from small plant samples.
  • Isolates total RNA including microRNAs.
  • No phenol/chloroform extraction or alcohol precipitation.
  • Isolate DNA-free plant RNA or RNA-free plant DNA

Other Notes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

Pictograms

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Signal Word

Warning

Hazard Statements

Precautionary Statements

RIDADR

UN 3316 9

Ira Marton et al.
Plant physiology, 154(3), 1079-1087 (2010-09-30)
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Protocols
The Plant/Fungi DNA Isolation Kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and fungi species.
Read More

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