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E7031

Sigma-Aldrich

Anti-EZH2 (N-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):
Anti-ENX-1, Anti-Enhancer of zeste homolog 2 (Drosophila)
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 100 kDa

species reactivity

human, mouse

technique(s)

western blot: 1:500-1:1,000 using K562 cell lysates
western blot: 1:500-1:1,000 using NIH3T3 cell lysates

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... EZH2(2146)
mouse ... Ezh2(14056)

General description

EZH2 gene is mapped to human chromosome 7q36.1.
Histone-lysine N-methyltransferase EZH2 is an enzyme encoded by the EZH2 gene in humans. It belongs to the multiprotein polycomb repressive complex 2.

Specificity

Anti-EZH2 (N-terminal) recognizes human and mouse EZH2.

Immunogen

synthetic peptide corresponding to amino acids 1-14 of human EZH2.

Application

Anti-EZH2 (N-terminal) antibody produced in rabbit has been used in chromatin immunoprecipitation (ChIP) and immunoblotting
Anti-EZH2 (N-terminal) antibody produced in rabbit is suitable for western blotting at a dilution of 1:500-1:1,000 using K562 cell lysates and NIH3T3 cell lysates.

Biochem/physiol Actions

Mutations in the EZH2 gene is implicated in the congenital disorder, Weaver syndrome.
Enhancer of zeste homologue 2 (EZH2) is crucially involved in epigenetic silencing by acting as a histone methyltransferase. It is overexpressed in many cancers and is involved in malignant cell proliferation and invasion. EZH2 may be associated with senescence and apoptosis in gastric cancer. It may act as a valuable biomarker for the diagnosis and prognosis of gastric cancer. EZH2 may have function in regulation of gene transcription and chromatin structure.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Gorica Nikoloski et al.
Nature genetics, 42(8), 665-667 (2010-07-06)
In myelodysplastic syndromes (MDS), deletions of chromosome 7 or 7q are common and correlate with a poor prognosis. The relevant genes on chromosome 7 are unknown. We report here that EZH2, located at 7q36.1, is frequently targeted in MDS. Analysis
Ana S A Cohen et al.
Human mutation, 37(3), 301-307 (2015-12-24)
Weaver syndrome (WS) is a rare congenital disorder characterized by generalized overgrowth, macrocephaly, specific facial features, accelerated bone age, intellectual disability, and susceptibility to cancers. De novo mutations in the enhancer of zeste homolog 2 (EZH2) have been shown to
Hanwen Zhang et al.
Oncogene, 39(5), 1041-1048 (2019-10-05)
Medulloblastoma (MB) is a malignant pediatric brain tumor for which new therapies are urgently needed. We demonstrate that treatment with EPZ-6438 (Tazemetostat), an enhancer of zeste homolog 2 (EZH2) inhibitor approved for clinical trials, blocks MB cell growth in vitro
Eri Imagawa et al.
Human mutation, 38(6), 637-648 (2017-02-24)
Weaver syndrome (WS) is a rare congenital overgrowth disorder caused by heterozygous mutations in EZH2 (enhancer of zeste homolog 2) or EED (embryonic ectoderm development). EZH2 and EED are core components of the polycomb repressive complex 2 (PRC2), which possesses
H Chen et al.
Genomics, 38(1), 30-37 (1996-11-15)
To identify genes that map on human chromosome 21 (HC21) and that may contribute to the phenotype of Down syndrome (DS), exon trapping was applied to cosmid DNA from an HC21-specific library LL21NCO2-Q. More than 550 potential exons were cloned

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