Skip to Content
MilliporeSigma

E8029

Sigma-Aldrich

E-Toxate™ Endotoxin standard

for endotoxin quantitation

Synonym(s):

E. coli 0.55:B5 LPS standard

Slide 1 of 1
Sign Into View Organizational & Contract Pricing

Select a Size

1 VIAL
$70.49

$70.49

List Price$74.20Save 5%
Web-Only Promotion

In StockDetails

Select a Size

Change View
1 VIAL
$70.49

About This Item

UNSPSC Code:
12352202
NACRES:
NA.24

Key Documents

View All Documentation

Skip To

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

$70.49

List Price$74.20Save 5%
Web-Only Promotion

In StockDetails

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Quality Level

200

contains

solubilizer

storage temp.

2-8°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
VT001796VT002023VT000126
E-Toxate™ Endotoxin standard for endotoxin quantitation

E8029

E-Toxate™ Endotoxin standard

Escherichia coli WDCM 00179 Vitroids™ 1,000-10,000 CFU mean value range, certified reference material, suitable for microbiology, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

VT001796

Escherichia coli WDCM 00179 Vitroids™

Escherichia coli WDCM 00202 Vitroids™ 80-130 CFU mean value range, certified reference material, suitable for microbiology, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

VT002023

Escherichia coli WDCM 00202 Vitroids™

Escherichia coli WDCM 00012 Vitroids™ 1,000-10,000 CFU mean value range, certified reference material, suitable for microbiology, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

VT000126

Escherichia coli WDCM 00012 Vitroids™

contains

solubilizer

contains

-

contains

-

contains

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

300

Quality Level

300

Quality Level

300

Application

E-Toxate endotoxin standard was used along with the limulus amebocyte lysate assay kit to determine the concentration of endotoxins in isolated samples of human holotransferrin and apotransferrin used to treat primary hepatocyte cultures. [1]

Other Notes

A preparation of E. coli 055:B5 lipopolysaccharide
Can be stored at 2-8 °C when stored separately from the E-Toxate kit.

Analysis Note

Standardized against USP reference standard endotoxin (RSE)

Legal Information

E-Toxate is a trademark of Sigma-Aldrich Co. LLC

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000429783
0000429783
0000397691
0000397691
0000331446

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

1–6 of 6 Questions  

  1. Hi, For E8029 endotoxin quantification product, 1. Is it a powder or liquid? 2. Solubility in water if it's a powder? How much water is needed to prepare a solution? Thanks,

    1 answer

    1. This product is a lyophilized powder typically used as a standard in the E-Toxate Endotoxin kits. See the link below to review these kits. For use with these kits, the material is reconstituted in endotoxin-free water at 4000UE/mL. Each vial contains a range of 10,000 - 20,000 units which is reported in the lot specific Certificate of Analysis. The mass weight is not determined. However, the amount of powder may be estimated based on the conversion factor of 5 EU per nanogram.
      Please see the link below to review a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/e8029#product-documentation

      Please see the link below to review the E-Toxate Endotoxin Kit products:
      https://www.sigmaaldrich.com/substance/etoxatekit1234598765

      Please see the link below to review the kit protocol with reconstitution instructions for this standard:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/378/800/et0200-pis-ms.pdf

      Helpful?

  2. What is the amount of the powder in each vial in grams or milligrams?

    1 answer

    1. The product comes as 10000 - 20000 EU Endotoxin per vial. Unfortunately, the exact weight information is not available.

      Helpful?

  3. What is the E-Toxate™ Endotoxin standard, Product E8029?

    1 answer

    1. The endotoxin standard is purified from a crude extract of lipopolysaccharide from E. coli 055:B5 and standardized against the current USP reference standard.  It is reconstituted with an appropriate amount of water to yield a 4000 EU/mL solution.

      Helpful?

  4. How much water should I use to reconstitute my E-Toxate™ Endotoxin standard, Product E8029?

    1 answer

    1. Each batch of endotoxin standard varies slightly in its activity so the amount of water to use may vary from lot to lot.  The certificate of analysis will give the amount of endotoxin units in the vial, so that the end user may calculate the appropriate amount of water to add to the vial in order to reconsitute to a final concentration of 4000 EU/mL.  The certificate of analysis for each lot may be retrieved from our website.

      Helpful?

  5. How is the E-Toxate™ Endotoxin standard, Product E8029, dissolved?

    1 answer

    1. E8029 is reconstituted in endotoxin-free water to a concentration of 4000 EU/mL.  Reconstituted standard solutions can be stored at 2 - 8°C for at least 2 weeks.

      Helpful?

  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service