This product is assigned a minimum activity specification of 50 units per milligram protein. At the minimum activity of 50 units, one vial would contain 20 ug of protein. The activity and protein content are lot specific and reported in the product Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/e8140#product-documentation
E8140
Elastase from human leukocytes
lyophilized powder, ≥50 units/mg protein (Bradford)
Synonym(s):
Lysosomal elastase
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 unit | Check Cart for Availability | $251.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥50 units/mg protein (Bradford)
Biological source:
human leucocytes
Quality Level
biological source
human leucocytes
form
lyophilized powder
specific activity
≥50 units/mg protein (Bradford)
mol wt
29 kDa
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... ELA2(1991), ELANE(1991)
General description
Leukocyte elastase is a 29 kDa serine endoprotease of the Proteinase S1 Family. It exists as a single 238 amino acid-peptide chain with four disulfide bonds. It contains two or thee N-linked glycans of variable composition which account for its three major isoforms.
Isoelectric point: pI = 8.77 - 9.55
Isoelectric point: pI = 8.77 - 9.55
Elastase is a proteolytic enzyme. It is a member of the subgroup named, peptidyl peptide hydrolases. It is a major anatomic constituent of arteries. It is mainly found in the pancreas and pancreatic juice of various birds and mammals. It is also present in human serum, granulocytes and erythrocytes.
Application
Elastase from human leukocytes has been used:
- to measure serum elastase activity
- in proteolytic digestion of fibronectin and salivary glands[1]
- in neutrophil elastase (NE) activity assay
- cell-free NE digestion of E-cadherin
- scratch wound assay
- in a study that determined that fragments of Nle3-angiotensin(1-7) accelerate healing in dermal models
Biochem/physiol Actions
Elastase enzyme is capable of releasing soluble peptides from insoluble elastin fibers with the help of a proteolytic process. It can stimulate disintegration of the axoneme with the help of adenosine triphosphate (ATP). Unlike pancreatic elastase the leukocyte enzyme has a preferential cleavage for the carboxyl side of valine, but will also cleave to a lesser extent after alanine. Natural substrates include elastin, cartilage proteoglycans, collagen types I, II, II and IV, and fibronectin.
Physical form
Lyophilized from 0.05 M sodium acetate (pH 5.5) and 0.6 M NaCl
Other Notes
One unit will release one nanomole of p-nitrophenol per sec from BOC-L-alanine p-nitrophenyl ester at pH 6.5 at 37 °C.
1 of 1
This Item | |||
|---|---|---|---|
| biological source human leucocytes | biological source - | biological source Porcine | biological source Porcine pancreas |
| specific activity ≥50 units/mg protein (Bradford) | specific activity ≥20 units/mg protein | specific activity ≥4 units/mg protein | specific activity ≥4.0 units/mg protein (biuret) |
| Gene Information human ... ELA2(1991), ELANE(1991) | Gene Information - | Gene Information - | Gene Information - |
| form lyophilized powder | form lyophilized solid | form lyophilized powder | form lyophilized powder |
| UniProt accession no. | UniProt accession no. - | UniProt accession no. - | UniProt accession no. - |
| shipped in dry ice | shipped in ambient | shipped in dry ice | shipped in - |
Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
QC Methods
Proteolytic Processing of the Epithelial Adherens Junction Molecule E-Cadherin by Neutrophil Elastase Generates Short Peptides With Novel Wound-Healing Bioactivity
Gordon MH, et al.
Cellular and molecular gastroenterology and hepatology, 7(2), 483-483 (2019)
Emily J Meyer et al.
Protein science : a publication of the Protein Society, 29(12), 2495-2509 (2020-10-22)
Corticosteroid-binding globulin (CBG) transports cortisol and other steroids. High-affinity CBG (haCBG) undergoes proteolysis of the reactive center loop (RCL) by neutrophil elastase (NE) altering conformation to low-affinity CBG (laCBG). Elevated temperature reduces CBG:cortisol binding affinity. Surface plasmon resonance was used
Yiming Shao et al.
Journal of cellular and molecular medicine, 26(7), 2089-2103 (2022-02-12)
Neutrophils release neutrophil extracellular traps (NETs) to capture and kill pathogens, but excessive NET release can damage the surrounding tissues. Myeloperoxidase (MPO) and neutrophil elastase (NE) are thought to be important in promoting histone depolymerization and DNA breakage in the
Global Trade Item Number
| SKU | GTIN |
|---|---|
| E8140-1UN | 04061833608630 |
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How much does each vial contains? please answer me using gram and not activity unit.
1 answer-
Helpful?
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Combien de temps et à quelle température peut-on stocker des aliquôts de solution l'élastase reconstituée dans le tampon pH5.5, s'il vous plaît?
1 answer-
This material is lyophilized from a solution containing 0.05 M sodium acetate (pH 5.5) and 0.6 M NaCl. The solution stability of this product has not been determined. However, reconstitution in water and snap-freezing in aliquots, then storing at -70°C, is recommended.
Helpful?
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Is this the active form of the enzyme? or does it require activation by cathepsin?
1 answer-
This product is not activated by cathepsin when tested for the activity of the enzyme. Please see the enzyme assay at the link below:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/506/826/e8140enz.pdfHelpful?
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How much enzyme is contained in a single package?
1 answer-
As provided, each vial contains one unit of Elastase enzyme activity, defined as the amount of enzyme required to catalyze the conversion of nanomole of p-nitrophenol per second from BOC-L-alanine p-nitrophenyl ester at pH 6.5 at 37 °C.
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