E8140

Sigma-Aldrich

Elastase from human leukocytes

lyophilized powder, ≥50 units/mg protein (Bradford)

Synonym(s):
Lysosomal elastase
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.54

form

lyophilized powder

Quality Level

specific activity

≥50 units/mg protein (Bradford)

mol wt

29 kDa

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

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General description

Elastase is a proteolytic enzyme. It is a member of the subgroup named, peptidyl peptide hydrolases. It is a major anatomic constituent of arteries. It is mainly found in the pancreas and pancreatic juice of various birds and mammals. It is also present in human serum, granulocytes and erythrocytes.

Application

Elastase from human leukocytes has been used:
  • to measure serum elastase activity
  • in proteolytic digestion of fibronectin and salivary glands
  • in neutrophil elastase (NE) activity assay
  • cell-free NE digestion of E-cadherin
  • scratch wound assay
  • in a study that determined that fragments of Nle3-angiotensin(1-7) accelerate healing in dermal models
Elastase has been used to digest fibronectin. The results were compared with fibronectin digestion by crude human leukocyte homogenate to examine the presence of fibronectin peptides in saliva of patients with Sjögren′s syndrome. It has also been used as a reference to determine the elastase activity in cell lysates. This study examined the effect of all-trans retinoic acid on procoagulant and fibrinolytic activities of cultured blast cells. These blast cells were from patients with acute promyelocytic leukemia.

Biochem/physiol Actions

Elastase enzyme is capable of releasing soluble peptides from insoluble elastin fibers with the help of a proteolytic process. It can stimulate disintegration of the axoneme with the help of adenosine triphosphate (ATP). Unlike pancreatic elastase the leukocyte enzyme has a preferential cleavage for the carboxyl side of valine, but will also cleave to a lesser extent after alanine. Natural substrates include elastin, cartilage proteoglycans, collagen types I, II, II and IV, and fibronectin.

Physical properties

Leukocyte elastase is a 29 kDa serine endoprotease of the Proteinase S1 Family. It exists as a single 238 amino acid-peptide chain with four disulfide bonds. It contains two or thee N-linked glycans of variable composition which account for its three major isoforms.
Isoelectric point: pI = 8.77 - 9.55

Unit Definition

One unit will release one nanomole of p-nitrophenol per sec from BOC-L-alanine p-nitrophenyl ester at pH 6.5 at 37 °C.

Physical form

Lyophilized from 0.05 M sodium acetate (pH 5.5) and 0.6 M NaCl

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Proteolytic Processing of the Epithelial Adherens Junction Molecule E-Cadherin by Neutrophil Elastase Generates Short Peptides With Novel Wound-Healing Bioactivity
Gordon MH, et al.
Cellular and molecular gastroenterology and hepatology, 7(2), 483-483 (2019)
Francisco J Silvestre et al.
Medicina oral, patologia oral y cirugia bucal, 14(8), e365-e370 (2009-03-21)
The purpose of this preliminary study was to monitor the degree of destruction of salivary glands in Sjögren disease by the detection of fibronectin peptides in patients' saliva. The sample consisted of 10 subjects divided in 2 groups, one with...
Maurits L van Montfoort et al.
Arteriosclerosis, thrombosis, and vascular biology, 33(1), 147-151 (2012-10-30)
The formation of neutrophil extracellular traps and the exposure of nucleosomes on these neutrophil extracellular traps contribute to coagulation activation and the propagation of deep vein thrombosis (DVT) in animal models. However, no data are available on the role of...
Benjamin J Staudinger et al.
American journal of respiratory and critical care medicine, 189(7), 812-824 (2014-01-29)
Progress has been made in understanding how the cystic fibrosis (CF) basic defect produces lung infection susceptibility. However, it remains unclear why CF exclusively leads to chronic infections that are noninvasive and highly resistant to eradication. Although biofilm formation has...
Mathieu Le Gars et al.
American journal of respiratory and critical care medicine, 187(2), 170-179 (2012-12-12)
Cystic fibrosis transmembrane conductance regulator (CFTR) protein is a chloride channel regulating fluid homeostasis at epithelial surfaces. Its loss of function induces hypohydration, mucus accumulation, and bacterial infections in CF and potentially other lung chronic diseases. To test whether neutrophil...

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