E8145

Sigma-Aldrich

Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid tetrasodium salt

≥97%

Synonym(s):
Egtazic acid tetrasodium, EGTA tetrasodium, Ethylene-bis(oxyethylenenitrilo)tetraacetic acid tetrasodium
Empirical Formula (Hill Notation):
C14H20N2O10Na4
CAS Number:
Molecular Weight:
468.28
MDL number:
PubChem Substance ID:
NACRES:
NA.77

Quality Level

assay

≥97%

solubility

H2O: soluble 10%, clear, colorless

SMILES string

[Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CCOCCOCCN(CC([O-])=O)CC([O-])=O)CC([O-])=O

InChI

1S/C14H24N2O10.4Na/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24;;;;/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24);;;;/q;4*+1/p-4

InChI key

BAOGCDPNNOBWQZ-UHFFFAOYSA-J

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Application

A chelating agent useful for the determination of calcium in the presence of magnesium. Repeated washing with EGTA-buffer can experimentally deplete cultured cells of Ca2+.
EGTA is suitable for use:
  • in media for chelating Ca2+ to facilitate efficient incorporation of Sr2+ into the oocytes
  • as a component of phosphatase wash buffer used to wash magnetic beads during dephosphorylation and radiolabeling of RNA segments cross-linked to immunoprecipitated proteins
  • as a component of oocyte activation media

Packaging

10, 50 g in glass bottle

Biochem/physiol Actions

Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetra acetic acid tetrasodium salt (EGTA) dissociates mucin complex and is useful in immunohistochemical studies. It suppresses root growth and mitotic index in germinating wheat seeds. It is used as a decalifying agent in root canal biomechanical preparation and is effective compared to EDTA and cyclohexanediaminetetraacetic acid (CDTA).
Ethylene glycol-bis(ß-aminoethyl ether)-N,N,N,N-tetra acetic acid tetrasodium salt (EGTA) is a calcium chelator that binds to Ca2+ in the presence of Mg2+ at a ratio of 1:1.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Satoshi Kishigami et al.
Methods in molecular biology (Clifton, N.J.), 1048, 109-126 (2013-08-10)
In mammals, a diploid genome following fertilization of haploid cells, an egg, and a spermatozoon is unique and irreproducible. This implies that the generated unique diploid genome is doomed with the individual's inevitable demise. Since it was first reported in...
Sayaka Wakayama et al.
Methods in enzymology, 476, 151-169 (2010-08-10)
Somatic cell nuclear transfer (SCNT) has become a unique and powerful tool for epigenetic reprogramming research and gene manipulation in animals. Although the success rates of somatic cloning have been inefficient and the mechanism of reprogramming is still largely unknown...
Schmid, R.W. et al.
Analytical Chemistry, 29, 264-264 (1957)
Sayaka Wakayama et al.
Methods in molecular biology (Clifton, N.J.), 530, 251-265 (2009-03-07)
We have been able to develop a stable nuclear transfer (NT) method in the mouse, in which donor nuclei are directly injected into the oocyte using a piezo-actuated micromanipulator. Although the piezo unit is a complex tool, once mastered it...
Shirley P and Saunders T L
Advanced Protocols For Animal Transgenesis, 271-277 (2011)

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