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E9762

Sigma-Aldrich

Endoglycosidase F1 from Elizabethkingia miricola

recombinant, expressed in E. coli, ≥16 U/mg, buffered aqueous solution

Synonym(s):
Endo F1, Endo-β-N-acetylglucosaminidase F1, Endoglycosidase F1 from Chryseobacterium meningosepticum, Endoglycosidase F1 from Elizabethkingia meningoseptica, Endoglycosidase F1 from Flavobacterium meningosepticum
Enzyme Commission number:
MDL number:
NACRES:
NA.32

recombinant

expressed in E. coli

Quality Level

conjugate

(N-linked)

form

buffered aqueous solution

specific activity

≥16 U/mg

mol wt

32 kDa

shipped in

wet ice

storage temp.

2-8°C

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General description

Endoglycosidase F1 from Elizabethkingia miricola is a glycan specific enzyme.

Application

Endoglycosidase F1 from Elizabethkingia miricola has been used to remove binding of human hemochromatosis protein (HFE) to cation independent mannose-6-phosphate receptor (CI-MPR) and to glycosylate fucosylated N-glycopeptides.
Cleaves asparagine-linked or free oligomannose and hybrid, but not complex, oligosaccharides.

Packaging

0.3, 1 unit
Supplied with 5× Reaction Buffer, 250 mM NaH2PO4, pH 5.5.

Biochem/physiol Actions

Endoglycosidase F1 from Elizabethkingia miricola plays an important role in cleavage of glycan structures from the protein by cleaving between the two N-acetylglucosamine residues of the chitobiose core. It mediates high mannose and hybrid oligosaccharides cleavage.

Unit Definition

One unit will release N-linked oligosaccharides from 1 μmole of denatured Ribonuclease B in 1 minute at 37 °C, pH 5.5.

Physical form

Aseptically filled solution in 20 mM Tris-HCl pH 7.5

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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