The key driver for yield in the GenElute-E kits is the fragment length, as this product utilizes a fractionation method. The expected yield should be greater than 80% as long as the sample is within a 50-100 µL load and greater than 50 bases.
EC800
GenElute™-E Single Spin RNA Cleanup Kit
Synonym(s):
GenElute™-E kits, Single Spin RNA Kit
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10 REACTIONS
$53.00
50 REACTIONS
$231.00
250 REACTIONS
$968.00
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10 REACTIONS
$53.00
50 REACTIONS
$231.00
250 REACTIONS
$968.00
About This Item
UNSPSC Code:
41105600
NACRES:
NA.52
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purified by
(Single-spin negative chromotography)
(Time: 3 minutes or less)
feature
Compatible Application (Suitable for most common downstream applications, including RT-PCR, gene expression, and NGS)
Intended use (For depletion of impurities from RNA solutions)
Typical/expected yield (Varies by sample. Please reference user guide for more information.)
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
greener alternative category
storage temp.
room temp
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This Item | EC700 | EC600 | EC300 |
|---|---|---|---|
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. - |
| purified by (Single-spin negative chromotography) | purified by (Single-spin negative chromotography), (Time: 3 minutes or less) | purified by (Single-spin negative chromotography), (Time: 3 minutes or less) | purified by (Single-spin negative chromotography), (Time: 45 minutes or less) |
| feature Compatible Application (Suitable for most common downstream applications, including RT-PCR, gene expression, and NGS) | feature Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Intended use (For the removal of organic solvent traces from DNA solutions), Typical/expected yield (Varies by sample. Please reference user guide for more information.) | feature Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Intended use (For depletion of impurities and partial fractions (<50 bp) from DNA solutions), Typical/expected yield (Varies by sample. Please reference user guide for more information.) | feature Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Intended use (For the purificiation of genomic DNA from human or animal tissue), Typical/expected yield (Varies by sample. Please reference user guide for more information.) |
| greener alternative product characteristics Waste Prevention | greener alternative product characteristics Waste Prevention | greener alternative product characteristics Waste Prevention | greener alternative product characteristics Waste Prevention |
| sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product |
| greener alternative category | greener alternative category , Aligned | greener alternative category | greener alternative category |
General description
DNA/RNA preparation by traditional methods frequently contains residual phenol and/or chloroform. The GenElute™-E Single Spin RNA Cleanup Kit is a one-step cleanup kit for the depletion of organic solvent traces, including phenol, TRIzol reagent, chloroform, and ethanol, from RNA solutions. Ideally suited for a reliable clean-up when a universal preparation for NGS library prep is desired or required, or for RNA cleanup following silica-based isolation to remove process contaminants
GenElute™-E kits are based on the principle of negative chromatography, where impurities like salt, detergent, proteins and organic solvents are removed by the column material and the purified nucleic acid is collected in the flow-through in just one single centrifugation step. This convenient procedure enables a significantly faster purification of nucleic acid samples than standard bind-wash-elute procedures, with less handling, drastically reduced plastic consumable usage, and no use of hazardous materials like chaotropic salts or organic solvents.
With conventional methods, traces of chaotropic salt and ethanol are usually coeluted with these purification procedures, which often leads to inhibition of real-time PCR experiments. GenElute™-E kits do not contain any chaotropic salts, organic solvents or EDTA, resulting in improved performance in downstream applications such as RT-PCR, gene expression, and NGS.
Because of the negative chromatography principle, there is no limitation to the amount of DNA that is cleaned up. Maximum sample volume is 100 μl. The purified nucleic acid is eluted in water and can immediately be used for downstream applications. A 1x TE Buffer, pH 8.5, is supplied to enable storage of samples.
GenElute™-E kits are based on the principle of negative chromatography, where impurities like salt, detergent, proteins and organic solvents are removed by the column material and the purified nucleic acid is collected in the flow-through in just one single centrifugation step. This convenient procedure enables a significantly faster purification of nucleic acid samples than standard bind-wash-elute procedures, with less handling, drastically reduced plastic consumable usage, and no use of hazardous materials like chaotropic salts or organic solvents.
With conventional methods, traces of chaotropic salt and ethanol are usually coeluted with these purification procedures, which often leads to inhibition of real-time PCR experiments. GenElute™-E kits do not contain any chaotropic salts, organic solvents or EDTA, resulting in improved performance in downstream applications such as RT-PCR, gene expression, and NGS.
Because of the negative chromatography principle, there is no limitation to the amount of DNA that is cleaned up. Maximum sample volume is 100 μl. The purified nucleic acid is eluted in water and can immediately be used for downstream applications. A 1x TE Buffer, pH 8.5, is supplied to enable storage of samples.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is aligned with "Waste prevention" and "Designing safer chemicals". Click here for more information.
Application
Traditional methods for purifying genomic RNA rely on organic solvents or medium and large format silica (bind-wash-elute) purification kits, which suffer from limited binding capacity, low RNA concentration, and low purity. The GenElute™-E Single Spin RNA Clean-up Kit, depletes impurities such as salts, detergents, nucleotides, primers, proteins/enzymes, organic solvents, and dyes from RNA solutions. Purified RNA is eluted in Tris buffer, pH 7.8 and can immediately be used for most common downstream applications, including RT-PCR, genotyping, NGS, and others.
Use within 12 months of receipt.
Features and Benefits
Fast and efficient purification (less than 3 minutes): single-spin protocol reduces pipetting steps and hands-on timeHigher quality RNA: negative chromotography-based purification reduces carryover of RT-PCR inhibitors and other contaminents Reduced environmental impact: 55% less plastic waste, as well as a significant reduction in hazardous chemicals
Other Notes
We believe that green chemistry will contribute to a better tomorrow. With a growing portfolio of greener alternatives, there are now more choices to reduce the ecological impact of your research while still delivering quality and efficacy so your results are not compromised. GenElute™-E purification kits avoid the use of hazardous wash buffers typically employed in silica-based purification kits, significantly reducing chemical waste. The single-spin protocol also limits the number of collection steps, reducing plastic waste by 55% compared to equivalent bind-wash-elute methods. GenElute™-E is designated a Greener Alternative Product based on the "Prevention" and "Designing Safer Chemicals" qualifications of The 12 Principles of Green Chemistry.GenElute™-E kits also adhere to the principles of SMASH Packaging, our global initiative to improve the sustainability through the reduction of packaging materials, the increased use of sustainable alternatives, and improved recyclability. Packaging is FCS-marked for sustainable forestry practices and produced from greater than 70% recycled content.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
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Is there a minimum value for using this kit? I have eight samples with RNA concentrations ranging from 5 to 14 ug. Does the kit work for me? How much RNA will lose during the procedure?
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