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EDEGLY

Sigma-Aldrich

Enzymatic Protein Deglycosylation Kit

Synonym(s):
Glycosylation Release Kit
NACRES:
NA.32

conjugate

(N-linked)
(O-linked)

Quality Level

shipped in

wet ice

storage temp.

2-8°C

General description

Enzymatic protein deglycosylation kit contains all the enzymes and reagents required for the complete removal of N-linked and simple O-linked carbohydrates from glycoproteins. This kit is employed to cleave the complex Core 2 O-linked carbohydrates including those containing polylactosamine.

Application

Enzymatic Protein Deglycosylation Kit has been used for the deglycosylation of various enzymes.
Enzymatic protein deglycosylation kit has been used in:
  • protein deglycosylation
  • deglycosylation of tissue factor pathway inhibitor (TFPI) α and β
  • deglycosylation of native exo-β-(1,3)-glucanase EXG1

Features and Benefits

Enzymatic protein deglycosylation kit has the following features:
  • two or more mg of glycoprotein is deglycosylated
  • single reaction at neutral pH
  • native & denaturing procedures
  • no degradation of protein
  • O-linked sugars containing polysialic acid are eliminated
  • control glycoprotein provided

Kit Components Only

Product No.
Description

  • PNGase F 1 mL/vial

  • O-Glycosidase 20 μL

  • α-2(3,6,8,9)-Neuraminidase 20 μL

  • Fetuin Control .5 mg

  • 5× Reaction Buffer .2 mL

  • Denaturation Solution .1 mL

  • Triton X-100 .1 mL

  • β-1→4-Galactosidase, positionally specific 20 μL

  • β-N-Acetylglucosaminidase 20 μL

See All (9)

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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