Skip to Content
MilliporeSigma

EDEGLY

Enzymatic Protein Deglycosylation Kit

Synonym(s):

Glycosylation Release Kit

Slide 1 of 1
Sign In to View Organizational & Contract Pricing.

Select a Size

1 KIT

$1,300.00

$1,300.00

Check Cart for Availability

About This Item

NACRES:
NA.32
UNSPSC Code:
12352202

Key Documents

View All Documentation

Skip To

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

conjugate

(N-linked), (O-linked)

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
PP0510CS0008NDEGLY
Enzymatic Protein Deglycosylation Kit

Sigma-Aldrich

EDEGLY

Enzymatic Protein Deglycosylation Kit

GlycoProfile™ IV Chemical Deglycosylation Kit TFMS Deglycosylation System

Sigma-Aldrich

PP0510

GlycoProfile™ IV Chemical Deglycosylation Kit

Enzymatic Protein Biotinylation Kit for biotinylation of up to 20 mg target protein

Sigma-Aldrich

CS0008

Enzymatic Protein Biotinylation Kit

Native Protein Deglycosylation Kit

Sigma-Aldrich

NDEGLY

Native Protein Deglycosylation Kit

conjugate

(N-linked)

conjugate

-

conjugate

-

conjugate

(N-linked)

shipped in

wet ice

shipped in

-

shipped in

dry ice

shipped in

wet ice

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−70°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Enzymatic protein deglycosylation kit contains all the enzymes and reagents required for the complete removal of N-linked and simple O-linked carbohydrates from glycoproteins. This kit is employed to cleave the complex Core 2 O-linked carbohydrates including those containing polylactosamine.

Application

Enzymatic Protein Deglycosylation Kit has been used for the deglycosylation of various enzymes.[1][2][3]
Enzymatic protein deglycosylation kit has been used in:
  • protein deglycosylation[4]
  • deglycosylation of tissue factor pathway inhibitor (TFPI) α and β[5]
  • deglycosylation of native exo-β-(1,3)-glucanase EXG1[6]

Features and Benefits

Enzymatic protein deglycosylation kit has the following features:
  • two or more mg of glycoprotein is deglycosylated
  • single reaction at neutral pH
  • native & denaturing procedures
  • no degradation of protein
  • O-linked sugars containing polysialic acid are eliminated
  • control glycoprotein provided

Kit Components Only

Product No.
Description
  • PNGase F 1 mL/vial
  • O-Glycosidase 20 μL
  • α-2(3,6,8,9)-Neuraminidase 20 μL
  • Fetuin Control .5 mg
  • 5× Reaction Buffer .2 mL
  • Denaturation Solution .1 mL
  • Triton™ X-100 .1 mL
  • β-1→4-Galactosidase, positionally specific 20 μL
  • β-N-Acetylglucosaminidase 20 μL
See All (9)

related product

Product No.
Description
Pricing

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000377170
0000377170
SLCM3146
SLCN4952
SLCK4329

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Guillain-Barre syndrome: First description of a snake envenomation aetiology
Neil J, et al.
Journal of Neuroimmunology, 242(1-2), 72-77 (2012)
Julien Chapuis et al.
Human molecular genetics, 20(10), 2026-2036 (2011-03-02)
Alzheimer's disease (AD), the most common neurodegenerative disorder, is characterized by cerebral deposition of amyloid-β (Aβ), a series of peptides derived from the processing of the amyloid-β precursor protein (APP). To identify new candidate genes for AD, we recently performed
Angela Mika et al.
PLoS neglected tropical diseases, 5(9), e1331-e1331 (2011-10-08)
Pruritic scabies lesions caused by Sarcoptes scabiei burrowing in the stratum corneum of human skin facilitate opportunistic bacterial infections. Emerging resistance to current therapeutics emphasizes the need to identify novel targets for protective intervention. We have characterized several protein families
Jonathan Trapala et al.
Protein expression and purification, 176, 105718-105718 (2020-08-11)
Exo-inulinases are versatile enzymes that have gained attention in recent years due to their ability to hydrolyze linear and branched polyfructose chains found in inulines. Agavin, a branched inulin, is found in Agave plant, the raw matter to produce tequila.
David K Crockett et al.
International journal of peptides, 2012, 621329-621329 (2012-04-27)
Histoplasmosis is a severe dimorphic fungus infection, which is often difficult to diagnose due to similarity in symptoms to other diseases and lack of specific diagnostic tests. Urine samples from histoplasma-antigen-positive patients and appropriate controls were prepared using various sample
View All Related Papers

Articles

Glycoprotein Deglycosylation

Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.

Strategies for Deglycosylating N-Linked Glycans

Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.

Post Translational Modification

Protein modifications are crucial for disease study. Analysis methods are key.

Deglycosylation Kits

Find deglycosylation kits for your research. Review the different features of deglycosylation kits for glycoproteins, enzymatic proteins, and native proteins.

See All

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service