EDEGLY

Sigma-Aldrich

Enzymatic Protein Deglycosylation Kit

NACRES:
NA.32

Quality Level

shipped in

wet ice

storage temp.

2-8°C

Related Categories

Application

Enzymatic Protein Deglycosylation Kit has been used for the deglycosylation of various enzymes.

Kit Components Only

Product No.
Description

  • PNGase F 1 mL/vial

  • O-Glycosidase 20 μL

  • α-2(3,6,8,9)-Neuraminidase 20 μL

  • Fetuin Control .5 mg

  • 5× Reaction Buffer .2 mL

  • Denaturation Solution .1 mL

  • Triton X-100 .1 mL

  • β-1→4-Galactosidase, positionally specific 20 μL

  • β-N-Acetylglucosaminidase 20 μL

See All (9)

Signal Word

Danger

RIDADR

UN 3316 9

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Jonathan Trapala et al.
Protein expression and purification, 176, 105718-105718 (2020-08-11)
Exo-inulinases are versatile enzymes that have gained attention in recent years due to their ability to hydrolyze linear and branched polyfructose chains found in inulines. Agavin, a branched inulin, is found in Agave plant, the raw matter to produce tequila....
Guillain-Barre syndrome: First description of a snake envenomation aetiology
Neil J, et al.
Journal of Neuroimmunology, 242(1-2), 72-77 (2012)
Angela Mika et al.
PLoS neglected tropical diseases, 5(9), e1331-e1331 (2011-10-08)
Pruritic scabies lesions caused by Sarcoptes scabiei burrowing in the stratum corneum of human skin facilitate opportunistic bacterial infections. Emerging resistance to current therapeutics emphasizes the need to identify novel targets for protective intervention. We have characterized several protein families...
Primavera Alvarado et al.
Journal of microbiological methods, 175, 105981-105981 (2020-06-15)
The diagnosis of histoplasmosis depends on various approaches: direct clinical examination, fungus isolation from cultures of clinical samples, histopathological evaluation, and serological testing. In serodiagnostic assays, the Histoplasma capsulatum H and M antigenic glycoproteins have been extensively used. However, both...
Scabies mite peritrophins are potential targets of human host innate immunity
Mika A, et al.
PLoS Neglected Tropical Diseases, 5(9), e1331-e1331 (2011)
Articles
Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.
Read More
There is no enzyme comparable to PNGase F for removing intact O-linked sugars. Monosaccharides must be sequentially hydrolyzed by a series of exoglycosidases until only the Gal-b(1-3)-GalNAc core remains. O-Glycosidase can then remove the core structure intact with no modification of the serine or threonine residue.
Read More
Deglycosylation Kits
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.