EMS0001

PNGase Fast

recombinant, expressed in E. coli

• Synonym(s): N-Glycosidase F, PNGase F, Peptide N-glycosidase
• UNSPSC Code: 41131616

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• conjugate: (N-linked)
• grade: Proteomics Grade
• form: ready-to-use solution
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Peptide-N-glycosidase F (PNGase F) belongs to an enzyme family, that are mainly used for the deglycosylation of N-linked glycans.[1]

Application

PNGase Fast may be used to immobilize in order to perform deglycosylation.[1] It may also be used to immobilize onto methacrylate based monolithic support to release the N-linked carbohydrate moieties from glycoproteins.[2]

Biochem/physiol Actions

Peptide-N-glycosidase F (PNGase F) cleaves asparagine-linked high mannose,[3] hybrid and complex oligosaccharides from glycoproteins. It can also deaminate the asparagine to aspartic acid.[4] PNGase Fast enables complete and rapid deglycosylation of antibodies and immunoglobulin fusion proteins, as well as other glycoproteins, to be prepared for downstream chromatography or mass spectrometry analysis. PNGase Fast creates an optimized workflow, reducing processing time without compromising sensitivity or reproducibility.

Safety Information

• Storage Class: 10 - Combustible liquids