F0767

Sigma-Aldrich

Monoclonal Anti-Human IgG1−FITC antibody produced in mouse

clone 8c/6-39, purified immunoglobulin, buffered aqueous solution

Synonym(s):
Monoclonal Anti-Human IgG1 (Fc specific)
NACRES:
NA.46

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

8c/6-39, monoclonal

form

buffered aqueous solution

storage condition

protect from light

application(s)

dot immunobinding: 1:64
particle immunofluorescence: 1:32

isotype

IgG2a

conjugate

FITC conjugate

shipped in

dry ice

storage temp.

−20°C

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General description

The antibody is specific for the Fc portion of human IgG1 and is non-reactive with other IgG subclasses. This clone, also described as HP-6091, was found to exhibit a valuable profile of reactivity and specificity for human IgG1 by the IUIS/WHO study.
Human IgG consists of four subclasses (1-4) that can be recognized by antigen differences in their heavy chains. They constitute approximately 65, 30, 5 and 4% of the total IgG respectively. Each subclass has different biological and physiochemical properties. The IgG subclass may be preferentially produced in response to different antigens. For instance, antipolysaccharide responses are mainly of the IgG2 subclass, while protein antigens give rise to IgG1 and IgG3 antibodies. Lipopolysaccahrides stimulate an IgG2 response in PBL′s and an IgG1 response in the spleen. Human IgG1 is the predominant subclass of in vivo and in vitro produced anti-tetanus toxoid antibodies.
Monoclonal Anti-Human IgG1 (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Application

Monoclonal Anti-Human IgG1−FITC antibody produced in mouse has been used in flow cytometry. It has also been used in direct hemagglutination (HA) and hemagglutination inhibition

Biochem/physiol Actions

IgG1 and IgG3 have an ability to adhere to the mononuclear phagocytes. A disproportionate elevation of IgG1 has also been found in the cerebral spinal fluid of patients with multiple sclerosis. Examination of the distribution pattern of IgG subclasses in different types of diseases may provide insight into the immunological processes involved and may assist in the diagnosis of various disorders.

Physical form

Solution in 0.01 M sodium phosphate buffer, pH 8.0, containing 1% inactivated bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
J D Isaacs et al.
Clinical and experimental immunology, 110(2), 158-166 (1997-11-21)
We have investigated the biological and therapeutic properties of a humanized anti-CD4 MoAb, hIgG1-CD4, in patients with refractory psoriasis and rheumatoid arthritis (RA). hIgG1-CD4 is a modulating, non-depleting MoAb, which induced a first-dose reaction in most patients treated. It provided...
E Cocco et al.
British journal of cancer, 103(6), 812-819 (2010-08-12)
Uterine serous papillary adenocarcinoma (USPC) is a highly aggressive variant of endometrial cancer. Human immuno-conjugate molecule (hI-con1) is an antibody-like molecule targeted against tissue factor (TF), composed of two human Factor VII (fVII) as the targeting domain, fused to human...
hI-con1, a factor VII-IgGFc chimeric protein targeting tissue factor for immunotherapy of uterine serous papillary carcinoma
Cocco E, et al.
British Journal of Cancer, 103 (2010)
Tissue factor expression in ovarian cancer: implications for immunotherapy with hI-con1, a factor VII-IgGFc chimeric protein targeting tissue factor
Cocco E, et al.
Clinical & Experimental Metastasis, 28 (2011)
Jocelyn-S Fraser et al.
World journal of gastroenterology, 12(48), 7805-7809 (2007-01-05)
To assess the level of undiagnosed coeliac disease (CD) in relatives of patients affected by the condition. We collected blood from 914 relatives of probands. We screened these individuals by ELISA for IgA and IgG tTG antibodies, confirming any positive...
Articles
Antibody-based serology tests are useful in identifying subjects with an adaptive immune response to the SARS-CoV-2 virus. Anti-human immunoglobulin antibodies allow for quick and simple, yet reliable assays with easy readouts and can also be adapted for high-throughput screening.
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