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F0895

Sigma-Aldrich

FIBRONECTIN FROM HUMAN PLASMA

liquid, 0.1% (Solution), BioReagent, suitable for cell culture

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Synonym(s):
Fibronectin
MDL number:
eCl@ss:
42020141
NACRES:
NA.75

biological source

human plasma

Quality Level

sterility

sterile

product line

BioReagent

form

liquid

packaging

pkg of 1 mg
pkg of 2 mg
pkg of 5 mg

concentration

0.1% (Solution)

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm2

solubility

saline: 0.05 M (Tris-buffered)

UniProt accession no.

binding specificity

Peptide Source: Collagen

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... FN1(2335)

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This Item
F2006F1056F0556
vibrant-m

F0895

FIBRONECTIN FROM HUMAN PLASMA

vibrant-m

F2006

Fibronectin human plasma

vibrant-m

F1056

Fibronectin human plasma

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

biological source

human plasma

biological source

human plasma

biological source

human plasma

biological source

human fibroblasts

sterility

sterile

sterility

sterile

sterility

sterile

sterility

sterile-filtered

concentration

0.1% (Solution)

concentration

>1 mg/mL protein

concentration

-

concentration

~0.5 mg/mL

UniProt accession no.

P02751

UniProt accession no.

P02751

UniProt accession no.

P02751

UniProt accession no.

P02751

General description

Fibronectin 1 is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. The FN1 gene mapped to human chromosome 2q35.
FN occurs as a dimeric protein, linked by two disulfide bonds at the end of C-terminal. Within the fibronectin structure, the domains are compactly arranged close to both the amino and carboxyl terminal of the peptide chains. Moreover, it has been observed that despite arising from a single gene up to 20 distinct variants of this gene have been identified in humans.

Application

Fibronectin from human plasma has been used in the determination of H2O2 consumption rate. It has also been used in coating the Sylgard® models for cell culture. It has also been used in cell adhesion and to directly pattern the protein onto the polymeric film.

Biochem/physiol Actions

Fibronectin function encompasses a diverse range of biological processes including cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Fibronectin protein plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis. Mutations in FN1 results in glomerulopathy.

Features and Benefits

  • Adhesive glycoprotein
  • Derived from human plasma
  • Recommended surface coverage of 1-5 μg/cm2

Preparation Note

This product is a 0.1% solution of fibronectin. The protein homogeneity is evaluated by immunoelectrophoresis, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C.

In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C

Analysis Note

Protein homogeneity is evaluated by immunoelectrophoresis.

Legal Information

Sylgard is a registered trademark of The Dow Chemical Company or an affiliated company of Dow

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

related product

Product No.
Description
Pricing

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Articles

Fibronectin (FN) is a multifunctional, extracellular plasma glycoprotein produced by hepatocytes that circulates at near micromolar concentration and assembles into extracellular matrix fibrils at cell surfaces along with locally produced cellular FN.

Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.

The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

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Protocols

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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