All Photos(5)

F0916

Sigma-Aldrich

Monoclonal Anti-Fibronectin antibody produced in mouse

clone IST-4, ascites fluid

MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

IST-4, monoclonal

mol wt

antigen 240 kDa

contains

15 mM sodium azide

species reactivity

human

should not react with

rabbit, bovine, chicken, mouse, goat, canine

technique(s)

immunocytochemistry: 1:100 using cultured human fibroblasts
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
indirect ELISA: suitable
radioimmunoassay: suitable
western blot: 1:250 using human fibroblasts cell extract

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... FN1(2335)

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General description

Mouse monoclonal clone IST-4 anti-Human Fibronectin antibody recognizes an epitope located within the 5th type III repeat of human plasma fibronectin. This epitope is common to all fibronectin forms. The antibody localizes plasma and cellular fibronectin both in its native and denatured-reduced forms (240 kDa) applying immunoblotting, RIA, ELISA, immunocytochemistry and immunohistochemistry. The product reacts with human fibronectin. No reactivity is observed with other species including goat, bovine, dog, rabbit, mouse and chicken.
Fibronectin 1 is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35.
Fibronectin (FN) is an extracellular matrix protein composed of two nearly-identical disulfide-bound polypeptides with typical molecular weights of 220-280 kDa. Cellular fibronectin is structurally and antigenically similar to cold insoluble globulin from plasma and antibodies to either form usually cross-react. Careful analysis of the FN molecule indicates that it contains several functionally and structurally distinct domains which may bind to cell surfaces and to a variety of molecules such as collagen, heparin, gelatin, fibrin and DNA. FN′s play an important role in diverse biological phenomena including cell adhesion, cell migration, hemostasis and thrombosis, wound healing and the ability to induce a more normal phenotype in transformed cells.

Immunogen

human plasma fibronectin

Application

Monoclonal Anti-Fibronectin antibody has been used :
  • in immunohistochemistry
  • in confocal microscopy
  • in immunocytochemistry
  • western blotting analysis

Mouse monoclonal clone IST-4 anti-Human Fibronectin antibody may be used for the specific labeling of fibronectin in cultured cells and in frozen tissue sections. Staining of formalin-fixed, paraffin-embedded sections may require prolonged proteolytic unmasking (e.g., by pepsin but not by trypsin).
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

Biochem/physiol Actions

Fibronectin participates in growth, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in the formation of extracellular matrix.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

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