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F1056

Sigma-Aldrich

Fibronectin human plasma

lyophilized powder, BioReagent, suitable for cell culture, ≥95% (SDS-PAGE)

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Synonym(s):
Fibronectin
EC Number:
MDL number:
NACRES:
NA.75

biological source

human plasma

Quality Level

sterility

sterile

product line

BioReagent

assay

≥95% (SDS-PAGE)

form

lyophilized powder

packaging

pkg of 1 mg
pkg of 2 mg
pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm2

solubility

H2O: 1 mg/mL at 37 °C

UniProt accession no.

binding specificity

Peptide Source: Collagen

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... FN1(2335)

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This Item
F2006F0556F0162
Fibronectin human plasma lyophilized powder, BioReagent, suitable for cell culture, ≥95% (SDS-PAGE)

F1056

Fibronectin human plasma

Fibronectin human plasma lyophilized powder, BioReagent, suitable for cell culture

F2006

Fibronectin human plasma

sterility

sterile

sterility

sterile

sterility

sterile-filtered

sterility

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

assay

≥95% (SDS-PAGE)

assay

≥85% (SDS-PAGE)

assay

-

assay

≥90% (SDS-PAGE)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

solution

form

lyophilized powder

General description

Fibronectin is a class of high-molecular-weight adhesive glycoprotein, predominant in extracellular matrix (ECM) and is widely distributed in tissue and blood. The FN gene, which is highly conserved, gives rise to a minimum of 20 isoforms through alternative splicing in humans. These isoforms include notable variants such as cellular FN and plasma FN. Plasma FN is primarily synthesized in the liver, whereas cellular FN which represents only 5% of the circulating fibronectins is locally produced by various cell types.

Application

Fibronectin human plasma has been used:
  • in neural selection medium to coat tissue culture surface for neuronal differentiation
  • to coat culture vessels for characterization of iPSC and iPSC‑derived BMEC in 2D cell culture
  • to coat culture supports for endothelial cell culture

Biochem/physiol Actions

Fibronectin plays a major role in biological processes. It interacts with surface receptors and other major ECM proteins like collagen to mediate cellular signals. Fibronectin is involved in a variety of cellular functions, including adhesion, cytoskeletal organization, oncogenic transformation, cell migration, phagocytosis, hemostasis, and embryonic differentiation. This glycoprotein can bind to integrins, collagens, fibrins and glycosaminoglycans (GAGs). FN plays a crucial role in promoting cell cycle progression and proliferation. This interaction influences cyclins and immediate early response genes, contributing to cellular processes related to cell division and growth. FN is also involved in cell survival and protection from apoptosis in various cell types, including epithelial and endothelial cells and leukocytes. FN serves as a critical factor in promoting cell survival and preventing programmed cell death in these cellular contexts. Mutations in the FN gene are associated with FN glomerulopathy and spondylometaphyseal dysplasia. Fibronectin plays a significant role in the wound healing process, and in extracellular matrix (ECM) assembly, hence is considered suitable for tissue engineering applications.

Caution

The reconstituted solution should be stored in working aliquots at -20°C or lower. Vortexing, excessive agitation, or additoinal freezing and thawing of reconstituted fibronectin are not recommended.

Preparation Note

This product is lyophilized from 0.05 M Tris buffered saline at pH 7.5. It is soluble at 1 mg/mL in water, and should be allowed to dissolve for at least 30 minutes at 37°C. A small amount of undissolved material may remain, but it will not affect performance. The protein homogeneity is evaluated by immunoelectrophoresis, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C.

In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Fibronectin
Fabrizio M and Angela S
Encyclopedia of Immunology, 909-913 (1998)
Floriana Burgio et al.
Cellular and molecular neurobiology, 43(8), 4173-4187 (2023-09-12)
Delivering biologics to elicit a therapeutic response in the central nervous system (CNS) remains challenging due to the presence of the blood-brain barrier (BBB). Receptor-mediated transcytosis is a strategy to improve brain exposure after systemic drug administration. The availability of
Jianfei He et al.
Redox biology, 36, 101631-101631 (2020-08-19)
Anastellin (AN), a fragment of the first type III module in fibronectin (FN), initiates formation of superfibronectin, a polymer which resembles the native cell-derived fibrillar FN found in the extracellular matrix of many tissues, but which displays remarkably different functional
Tests to predict preeclampsia
Agustin CA, et al.
Journal of Separation Science (2015)
Fibronectin
Breisnes HW, et al.
Biochemistry of Collagens, Laminins and Elastin (2024)

Articles

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Protocols

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

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