F1180

Sigma-Aldrich

Anti-Flotillin 1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-Reggie-2, Anti-FLOT1
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

~48 kDa

species reactivity

rat, mouse, human

concentration

~1 mg/mL

application(s)

indirect immunofluorescence: 5-10 μg/mL using human HEK-293K cells
western blot: 0.5-1 μg/mL using whole extract of mouse lung
western blot: 1-2 μg/mL using whole extract of rat lung

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... FLOT1(10211)
mouse ... Flot1(14251)
rat ... Flot1(64665)

General description

Flotillin 1(FLOT1), also known as reggie-2, is an estrogen responsive gene mapped to human chromosome 6p21.33. This gene codes for an integral membrane protein and a component of lipid rafts.
Flotillins are lipid raft associated proteins expressed mainly in adipose tissue, striated muscle tissues, and lung. It has a crucial role in several cellular processes like phagocytosis, endocytosis, insulin signalling and neuronal regeneration. Anti-flotillin 1 antibody can be used in immunobloting and immunolocalization. Rabbit anti- flotillin 1 antibody reacts specifically with flotillin 1 of rat, mouse and human.

Immunogen

synthetic peptide corresponding to amino acid resides 413-428 of mouse flotillin 1 with an N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in rat and differs by one amino acid in human.

Application

Anti-flotillin 1 antibody produced in rabbit has been used in immunoblot analysis.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Western Blotting (1 paper)
Anti-flotillin 1 antibody can be used in immunofluorescence and western blotting.

Biochem/physiol Actions

Flotillin 1 (FLOT1) has a role in endocytosis. It also functions in growth factor signaling and activates mitogen-activated protein kinase signaling. In addition, it is also involved in phagocytosis, regulation of actin cytoskeleton and membrane trafficking. The protein has been associated with tumor progression and is expressed in many cancers, including breast cancer.

Target description

Flotillin 1 encodes a caveolae-associated, integral membrane protein. Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 3

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Takeshi Yamasaki et al.
The Journal of general virology, 93(Pt 3), 668-680 (2011-11-18)
Generation of an abnormal isoform of the prion protein (PrP(Sc)) is a key aspect of the propagation of prions. Elucidation of the intracellular localization of PrP(Sc) in prion-infected cells facilitates the understanding of the cellular mechanism of prion propagation. However...
Aubin Moutal et al.
Pain, 162(1), 243-252 (2020-10-04)
Global spread of severe acute respiratory syndrome coronavirus 2 continues unabated. Binding of severe acute respiratory syndrome coronavirus 2's spike protein to host angiotensin-converting enzyme 2 triggers viral entry, but other proteins may participate, including the neuropilin-1 receptor (NRP-1). Because...
Carsten Frühbeis et al.
Journal of extracellular vesicles, 4, 28239-28239 (2015-07-05)
Cells secrete extracellular vesicles (EVs) by default and in response to diverse stimuli for the purpose of cell communication and tissue homeostasis. EVs are present in all body fluids including peripheral blood, and their appearance correlates with specific physiological and...
Panagiotis Papatheodorou et al.
mBio, 4(3), e00244-e00213 (2013-05-02)
Clostridium difficile is the leading cause of antibiotics-associated diarrhea and pseudomembranous colitis. Hypervirulent C. difficile strains produce the binary actin-ADP-ribosylating toxin CDT (C. difficile transferase), in addition to the Rho-glucosylating toxins A and B. We recently identified the lipolysis-stimulated lipoprotein...
Ilse Delint-Ramirez et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 30(24), 8162-8170 (2010-06-18)
Lipid rafts are dynamic membrane microdomains enriched in cholesterol and sphingolipids involved in the compartmentalization of signaling pathways, trafficking and sorting of proteins. At synapses, the glutamatergic NMDA receptor and its cytoplasmic scaffold protein PSD-95 move between postsynaptic density (PSD)...

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