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F1262

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment–FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

MDL number:

Quality Level

biological source

goat

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

application(s)

indirect immunofluorescence: 1:40

conjugate

FITC conjugate

storage temp.

2-8°C

General description

Goat polyclonal anti-Rabbit IgG (whole molecule), F(ab′)2 fragment–FITC antibody binds all rabbit IgGs.
Immunoglobulins (Igs) are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs can be useful tools for the analysis of target proteins
Anti-Rabbit IgG (whole molecule), (F(ab′)2) fragment-FITC is specific for all rabbit immunoglobulins. The use of this product prevents background staining due to the presence of Fc receptors.
Rabbit IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rabbit serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.

Immunogen

Purified rabbit IgG

Application

Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment–FITC antibody produced in goat has also been used for fluorescence labeling and immunocytochemistry.
Anti-Rabbit IgG (whole molecule), (F(ab′)2) fragment-FITC is suitable for use in flow cytometry.
Goat polyclonal anti-Rabbit IgG (whole molecule), F(ab′)2 fragment–FITC antibody may be used to detect and quantitate the level of IgG in rabbut serum and biological fluids by fluorescent techniques. It may also be used as a secondary antibody in assays that use rabbit IgG as the primary antibody. F(ab′)2 fragment–FITC antibody is useful when trying to avoid background staining due to the presence of Fc receptors.

Packaging

0.5 mL in glass insert
1, 2 mL in glass bottle

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Differentiation of human alveolar epithelial cells in primary culture: morphological characterization and synthesis of caveolin-1 and surfactant protein-C
Fuchs S, et al.
Cell and Tissue Research, 311(1), 31-45 (2003)
Edith Terrenoire et al.
BMC genetics, 16, 44-44 (2015-05-01)
Using metaphase spreads from human lymphoblastoid cell lines, we previously showed how immunofluorescence microscopy could define the distribution of histone modifications across metaphase chromosomes. We showed that different histone modifications gave consistent and clearly defined immunofluorescent banding patterns. However, it...
Synergistic induction of PI-PLCβ1 signaling by azacitidine and valproic acid in high-risk myelodysplastic syndromes
Follo MY, et al.
Leukemia, 25(2), 271-271 (2011)
U Olszewski-Hamilton et al.
Biomarkers in cancer, 3, 31-40 (2011-01-01)
The role of organic anion transporting polypeptide 5A1 (OATP5A1) a member of a family of drug transporters that mediate cellular uptake of drugs has not been characterized so far. Gene expression levels of OATP5A1 in small cell lung cancer (SCLC)...
Giovanni Barillari et al.
Oncology letters, 12(4), 2389-2394 (2016-10-05)
The infection of uterine cervical epithelial cells by oncogenic, high-risk human papilloma viruses (HR-HPVs) may lead to the development of cervical carcinoma. Of note, the incidence of this tumor is significantly increased in women infected by both HR-HPV and human...

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