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F1418

Sigma-Aldrich

Ficoll® 400

BioXtra, Type 400-DL, lyophilized powder

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Synonym(s):
Polysucrose 400
CAS Number:
MDL number:
NACRES:
NA.25

type

Type 400-DL

product line

BioXtra

form

lyophilized powder

impurities

≤0.0005% Phosphorus (P)
≤0.1% Insoluble matter

ign. residue

<0.1%

color

white

solubility

water: soluble

anion traces

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

cation traces

Al: ≤0.0005%
Ca: ≤0.001%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.001%
NH4+: ≤0.05%
Na: ≤0.01%
Pb: ≤0.001%
Zn: ≤0.0005%

storage temp.

15-25°C

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1 of 4

This Item
F8636F2637F9378
Ficoll&#174; 400 BioXtra, Type 400-DL, lyophilized powder

Sigma-Aldrich

F1418

Ficoll® 400

Premium Grade
Sigma-Aldrich

Sigma-Aldrich

F8636

Ficoll® 400

-
Sigma-Aldrich

Sigma-Aldrich

F2637

Ficoll® 400

-
Ficoll&#174; 400 Type 400-DL, lyophilized powder

Sigma-Aldrich

F9378

Ficoll® 400

-
form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

impurities

≤0.0005% Phosphorus (P), ≤0.1% Insoluble matter

impurities

-

impurities

-

impurities

-

color

white

color

-

color

-

color

white

solubility

water: soluble

solubility

-

solubility

-

solubility

H2O: soluble 100 mg/mL, clear to slightly hazy, colorless to faintly yellow

cation traces

Al: ≤0.0005%, Ca: ≤0.001%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Mg: ≤0.001%, NH4+: ≤0.05%, Na: ≤0.01%, Pb: ≤0.001%, Zn: ≤0.0005%

cation traces

-

cation traces

-

cation traces

-

Application

Ficoll® 400 is used together with sodium diatrizoate (Paque) to create Ficoll-Paque, a density mixture, used for the separation of cells and organelles, especially lymphocytes and monocytes from blood. Ficoll 400® may be used for the development of new cell and organelle separation systems. Ficoll 400 is used in tissue engineering and macromolecular conformation research for the development, evaluation and use of macromolecular crowding (MMC) systems and configurations.
Used for cell separation and organelle isolation.

Other Notes

A nonionic synthetic polymer of sucrose.
To gain a comprehensive understanding of our extensive range of Polysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Legal Information

Ficoll is a registered trademark of Cytiva

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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Soluble form of the endothelial adhesion molecule CD146 binds preferentially CD16+ monocytes.
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Protocols

Ficoll 400® is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400® is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400® is very hydrophilic and extremely water-soluble.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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