F1554

Sigma-Aldrich

Anti-FXR2 antibody, Mouse monoclonal

clone A42, purified from hybridoma cell culture

MDL number:
NACRES:
NA.41
Pricing and availability is not currently available.

Quality Level

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

A42, monoclonal

form

buffered aqueous solution

mol wt

antigen ~74 kDa

species reactivity

human

application(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 2-4 μg/mL using total cell extract of HEK 293T cells

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... FXR2(9513)

General description

Monoclonal Anti-FXR2 (mouse IgG1 isotype) is derived from the hybridoma A42 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with human Fragile X mental retardation syndrome-related protein 2 (FXR2) recombinant protein. FXR2 gene is located on human chromosome 17 and its protein is localized mainly in the cytoplasm. FXR2 is expressed along with other proteins in the cytoplasm of neurons. It is characterized with two KH domains and one RGG box that together with fragile X mental retardation 1 (FMR1) bind to RNA. FXR2 is highly expressed in brain and testis.

Specificity

Monoclonal Anti-FXR2 recognizes human, monkey, bovine, canine, rat, hamster, and mouse, FXR2, ~74 kDa, and does not crossreact with FMR or FXR1.

Immunogen

human FXR2 recombinant protein.

Application

Anti-FXR2 antibody, Mouse monoclonal may be used in:
  • immunoblotting
  • immunoprecipitation
  • immunocytochemistry
  • immunohistochemistry
  • co-immunoprecipitation
  • immunofluorescence
  • sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)

Biochem/physiol Actions

Fragile X mental retardation syndrome-related protein 2 (FXR2) knockout mice are hyperactive in the open-field test, impaired on the rotarod test, have reduced levels of prepulse inhibition, display less contextual conditioned fear, are impaired at locating the hidden platform in the Morris water task, and less sensitive to heat stimulus.
Fragile X mental retardation syndrome-related protein 2 (FXR2) is capable of forming heteromers with the others and can also form homomers. It has an independent function in tissues during embryonic development and adult life.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For prolonged storage, freeze in working aliquots. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Fragile X granules are a family of axonal ribonucleoprotein particles with circuit-dependent protein composition and m RNA cargos
Chyung E, et al.
The Journal of Comparative Neurology, 526(1), 96-108 (2018)
Knockout mouse model for Fxr2: a model for mental retardation
Bontekoe CJM, et al.
Human Molecular Genetics, 11(5), 487-498 (2002)
Widespread RNA editing dysregulation in brains from autistic individuals
Tran SS, et al.
Nature Neuroscience, 22(1), 25-25 (2019)
FXR1 regulates transcription and is required for growth of human cancer cells with TP53/FXR2 homozygous deletion
Fan Y, et al.
eLife, 6, e26129-e26129 (2017)
D R Joseph
Steroids, 63(1), 2-4 (1998-01-23)
The rat androgen-binding protein/sex hormone-binding globulin (ABP/SHBG) gene is regulated by promoters P1 and PA. P1 regulates the mRNA encoding secreted ABP/SHBG, whereas PA regulates an alternate mRNA which encodes a modified protein that is targeted to the nucleus. Promoter...

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