F1802

Sigma-Aldrich

Fibroblast Growth Factor-8c from mouse

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

Synonym(s):
FGF-8c
MDL number:
Pricing and availability is not currently available.

biological source

mouse

recombinant

expressed in E. coli

assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

10-50 ng/mL ED50

mol wt

apparent mol wt 31 kDa by SDS-PAGE
predicted mol wt 28 kDa

packaging

pkg of 25 μg

storage condition

avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)

impurities

endotoxin, tested

UniProt accession no.

storage temp.

−20°C

Gene Information

mouse ... Fgf8(14179)

General description

Fgf8 (fibroblast growth factor 8) is a member of the FGF family of proteins. This family was originally identified as fibroblast mitogen in bovine pituitary and brain. Both humans and mice, have six to seven subfamilies of FGF protein containing 22 members. FGF gene is composed of three exons, and the first exon is alternatively spliced to produce four variants. This gene is expressed in embryonic organs such as brain, eye, ear, hearts and limbs.

Application

Fibroblast Growth Factor-8c from mouse is suitable for the prevention of cross-reaction in Western blot and SDS-PAGE for FGF2 protein.

Biochem/physiol Actions

Fgf8 (fibroblast growth factor 8) plays an essential role in the development of dorsal raphe nucleus (DR), and is expressed in fetus in the developing anterior hindbrain. DR serotonergic neurons are born in this area of the brain, and this is achieved due to the formation of diffusion gradient by the secreted Fgf8 peptide. Failure of this results in loss of serotonergic neurons during brain development. A nonsense mutation in this gene is linked with isolated hypogonadotropic hypogonadism (IHH), with different degrees of olfactory phenotype and human gonadotropin-releasing deficiency.
FGF-8c plays a role in ectodermal differentiation in the post-gastrulation embryo, including outgrowth and patterning of the face, limbs, and central nervous system.

Physical form

Lyophilized from 0.2 μm filtered solution in phosphate buffered saline containing 1.25 mg bovine serum albumin.

Analysis Note

The biological activty is measured by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.

RIDADR

NONH for all modes of transport

WGK Germany

3

Bettina A Moser et al.
The EMBO journal, 28(7), 810-820 (2009-02-14)
To maintain genomic integrity, telomeres must undergo switches from a protected state to an accessible state that allows telomerase recruitment. To better understand how telomere accessibility is regulated in fission yeast, we analysed cell cycle-dependent recruitment of telomere-specific proteins (telomerase...
N Abimbola Sunmonu et al.
Journal of cellular physiology, 226(7), 1722-1726 (2011-04-21)
Soluble growth factors play an important role in the coordination and integration of cell proliferation, differentiation, fate determination, and morphogenesis during development of multicellular organisms. Fibroblast growth factors (FGFs) are a large family of polypeptide growth factors that are present...
X Shi et al.
Oncogenesis, 4, e176-e176 (2015-12-01)
Sonic hedgehog (Shh) signaling is critical during normal development, and the abnormal activation of the Shh pathway is involved in many human cancers. As a target gene of the Shh pathway and as a transcription activator downstream of Shh signaling...
Tetsu M C Yung et al.
Experimental cell research, 315(10), 1693-1705 (2009-02-28)
Negative Elongation Factor (NELF) is a transcription factor discovered based on its biochemical activity to suppress transcription elongation, and has since been implicated in various diseases ranging from neurological disorders to cancer. Besides its role in promoter-proximal pausing of RNA...
Ericka B Trarbach et al.
The Journal of clinical endocrinology and metabolism, 95(7), 3491-3496 (2010-05-14)
FGFR1 mutations cause isolated hypogonadotropic hypogonadism (IHH) with or without olfactory abnormalities, Kallmann syndrome, and normosmic IHH respectively. Recently, missense mutations in FGF8, a key ligand for fibroblast growth factor receptor (FGFR) 1 in the ontogenesis of GnRH, were identified...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.