F1930

Sigma-Aldrich

Monoclonal Anti-hFBXW7γ antibody produced in mouse

~2 mg/mL, clone FB343, purified immunoglobulin, buffered aqueous solution

Synonym(s):
Anti-SEL-10 (C. elegance), Anti-ARCHIPELAGO (Drosophila), Anti-FBW7, Anti-F-box and WD40 domain protein 7, Anti-Cdc4 (S. serevisia)
MDL number:

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

FB343, monoclonal

form

buffered aqueous solution

mol wt

antigen ~60 kDa

species reactivity

human, mouse, rat

packaging

antibody small pack of 25 μL

concentration

~2 mg/mL

application(s)

immunocytochemistry: 10-20 μg/mL using human A431 cells
indirect ELISA: suitable
western blot: 5-10 μg/mL using human A431 cell extracts

isotype

IgG2a

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... FBXW7(55294)
mouse ... Fbxw7(50754)

Related Categories

General description

FBXW7 is a F-box protein that facilitates as a substrate recognition component of the ubiquitin ligase complex SCF (Skp-Cullin-F-box). FBXW7 exist in three isoforms α, β and γ. It is composed of a conserved F-box domain at their N terminus, which interacts with Skp1. C terminus consist of one or several highly variable protein-protein interaction domains such as Leu-rich repeat (LRR), kelch repeat, tetratricopeptide repeat (TPR), and WD40 repeat.

Immunogen

synthetic peptide corresponding to amino-acids 13-27 of human hFBXW7γ isoform.

Application

Monoclonal Anti-hFBXW7γ antibody is suitable for:-
  • Immunocytochemistry at a concentration of 10-20μg/mL using human A431 cells.
  • Western blot at a concentration of 5-10μg/mL using human A431 cell extracts.
  • Indirect ELISA.

Biochem/physiol Actions

FBXW7 protein is highly involved in the ubiquitination of cyclin E , Notch , c-Jun and c-Myc. It acts as a key component of phosphoepitope-specific substrate recognition complex of SCF ubiquitin ligases to facilitate the recognition of phosphorylated targets such as cyclin E, myc, c-Jun, and Notch. The SV40 large T antigen binds FBXW7 without detectible effects on its stability to acts as an inhibitor of FBXW7 activity.It has been reported that FBXW7 controls c-Myc′s growth-promoting activity followed by cell size, which is an important event of Fbw7 loss in cancers.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

3

Certificate of Analysis

Certificate of Origin

Mukesh Jain et al.
Plant physiology, 143(4), 1467-1483 (2007-02-13)
F-box proteins constitute a large family in eukaryotes and are characterized by a conserved F-box motif (approximately 40 amino acids). As components of the Skp1p-cullin-F-box complex, F-box proteins are critical for the controlled degradation of cellular proteins. We have identified...
Nicole E Willmarth et al.
Breast cancer research : BCR, 6(5), R531-R539 (2004-08-21)
Cyclin E, a G1 cyclin essential for G1-S phase transition, is known to have a profound effect on tumorigenesis. Elevated levels of cyclin E have been associated with breast cancer, and chromosomal instability observed in breast cancer is suggested to...
Markus Welcker et al.
The Journal of biological chemistry, 280(9), 7654-7658 (2004-12-22)
Cell transformation by simian virus 40 (SV40) results mostly from the highly oncogenic activities of the large T antigen (LT), which corrupts the cellular checkpoint mechanisms that guard cell division and the transcription, replication, and repair of DNA. The most...
Markus Welcker et al.
Current biology : CB, 14(20), 1852-1857 (2004-10-23)
The human tumor suppressor Fbw7/hCdc4 functions as a phosphoepitope-specific substrate recognition component of SCF ubiquitin ligases that catalyzes the ubiquitination of cyclin E , Notch , c-Jun and c-Myc . Fbw7 loss in cancer may thus have profound effects on...
Masayoshi Yada et al.
The EMBO journal, 23(10), 2116-2125 (2004-04-23)
The F-box protein Skp2 mediates c-Myc ubiquitylation by binding to the MB2 domain. However, the turnover of c-Myc is largely dependent on phosphorylation of threonine-58 and serine-62 in MB1, residues that are often mutated in cancer. We now show that...

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