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cell culture | mammalian: suitable
H2O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)
human ... FN1(2335)
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If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
The species source for the fibronectin used when coating culture surfaces is not important. It is the presence of the RGD binding site that is present in all fibronectins that promotes binding.
The product is good for at least a year at 2-8 °C if kept sterile.
Fibronectin coated cultureware can be stored for 2-4 weeks at 2-8 °C in a closed sterile container, or in sterile sealable bags.
To coat culture surfaces: 1. Dilute fibronectin in sterile balanced salt solution and coat the culture surface (1-5 μg/cm2) with a minimal volume. 2. Allow to air dry for at least 45 minutes at room temperature. Excess fibronectin may be removed by aspiration, but this is not necessary.
Dissolve at 1 mg/mL in water. Allow to dissolve for at least 30 minutes at 37 °C. A small amount of undissolved material may remain. This will not affect product performance.
We test the coated culture surface by culturing cells. We compare the adherence of the cells to wells that have been coated with media containing BSA (negative) and media containing 10% FBS (positive). The cell growth should be not less than 50% of the positive control and greater than 50% of the negative control.
As the product may contain undissolved material, the amount of fibronectin in solution can be determined by UV absorbance. The extinction coefficient for a 1% solution (10 mg/ml) is 13.5 at 280 nm.
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