F2426

EZview^™ Red ANTI-FLAG^® M2 Affinity Gel

clone M2

• Synonym(s): Monoclonal ANTI-FLAG^® M2 antibody produced in mouse, Anti-ddddk, Anti-dykddddk
• NACRES: NA.32

Properties

• clone: M2, monoclonal
• Quality Level: 200
• analyte chemical class(es): proteins (FLAG^® Affinity Gels, FLAG^® tag, 3xFLAG^® tag, DYKDDDDK tag)
• technique(s): affinity chromatography: suitable; immunoprecipitation (IP): suitable
• matrix: 4% agarose bead; 45-165μm bead size
• isotype: IgG1
• capacity: ≥0.6 mg/mL, gel binding capacity
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

EZ view Red Anti-FLAG M2 Affinity Gel is a resin that consists of Anti-FLAG M2 antibody, covalently bonded to 4% Agarose beads. The affinity gel is used to bind FLAG fusion proteins to samples, such as cell lysates and tissue, for purification of FLAG-tagged proteins in preparation of immunoprecipitation assays. Red dye enhances visability for more efficient results. Agarose beads bind at N-terminal, Met-N-terminal and C-terminal FLAG fusion proteins, 3xFLAG-tagged fusion proteins.

Specificity

Suitable for purifying N-terminal, Met-N-terminal, C-terminal FLAG fusion proteins, 3xFLAG fusion proteins.

Application

Immunoprecipitation (IP) of FLAG- and 3xFLAG-tagged fusion proteins.

Elution - FLAG peptide, Glycine, pH 3.5, 3x FLAG peptide

Browse additional application references in our FLAG^® Literature portal.

Physical form

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

EZview is a trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable