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EZview Red ANTI-FLAG® M2 Affinity Gel

clone M2

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Synonym(s):
Monoclonal ANTI-FLAG® M2 antibody produced in mouse, Anti-ddddk, Anti-dykddddk
NACRES:
NA.32

clone

M2, monoclonal

Quality Level

analyte chemical class(es)

proteins (FLAG® Affinity Gels, FLAG® tag, 3xFLAG® tag, DYKDDDDK tag)

technique(s)

affinity chromatography: suitable
immunoprecipitation (IP): suitable

matrix

4% agarose bead; 45-165μm bead size

isotype

IgG1

capacity

≥0.6 mg/mL, gel binding capacity

shipped in

wet ice

storage temp.

−20°C

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This Item
A2220F1804A9469
Millipore

A2220

ANTI-FLAG® M2 Affinity Gel

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

technique(s)

affinity chromatography: suitable (FLAG® peptide, Glycine, pH3.5, 3x FLAG® peptide), immunoprecipitation (IP): suitable

technique(s)

-

technique(s)

indirect ELISA: 1:20,000

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

isotype

IgG1

isotype

IgG1

isotype

IgG1

isotype

IgG1

analyte chemical class(es)

proteins (FLAG® Affinity Gels, FLAG® tag, 3xFLAG® tag, DYKDDDDK tag)

analyte chemical class(es)

proteins (FLAG® tag, 3x FLAG®, DYKDDDDK tag)

analyte chemical class(es)

-

analyte chemical class(es)

-

General description

EZ view Red Anti-FLAG M2 Affinity Gel is a resin that consists of Anti-FLAG M2 antibody, covalently bonded to 4% Agarose beads. The affinity gel is used to bind FLAG fusion proteins to samples, such as cell lysates and tissue, for purification of FLAG-tagged proteins in preparation of immunoprecipitation assays. Red dye enhances visability for more efficient results. Agarose beads bind at N-terminal, Met-N-terminal and C-terminal FLAG fusion proteins, 3xFLAG-tagged fusion proteins.

Specificity

Suitable for purifying N-terminal, Met-N-terminal, C-terminal FLAG fusion proteins, 3xFLAG fusion proteins.

Application

Immunoprecipitation (IP) of FLAG- and 3xFLAG-tagged fusion proteins.

Elution - FLAG peptide, Glycine, pH 3.5, 3x FLAG peptide

Learn more product details in our FLAG® application portal.

Physical form

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
EZview is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

related product

Product No.
Description
Pricing

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Kara R Jones et al.
Molecular cancer therapeutics, 4(10), 1541-1547 (2005-10-18)
Incomplete DNA repair or misrepair can contribute to the cytotoxicity of DNA double-strand breaks. Consequently, interference with double-strand break repair, by pharmacologic or genetic means, is likely to sensitize tumor cells to ionizing radiation. The current studies were designed to
Matthew S Walters et al.
Journal of virology, 84(13), 6861-6865 (2010-04-16)
Varicella zoster virus encodes an immediate-early (IE) protein termed ORF61p that is orthologous to the herpes simplex virus IE protein ICP0. Although these proteins share several functional properties, ORF61p does not fully substitute for ICP0. The greatest region of similarity
Jiahai Zhou et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(39), 14343-14348 (2006-09-16)
The unfolded protein response (UPR) is an evolutionarily conserved mechanism by which all eukaryotic cells adapt to the accumulation of unfolded proteins in the endoplasmic reticulum (ER). Inositol-requiring kinase 1 (IRE1) and PKR-related ER kinase (PERK) are two type I
W Du et al.
Cell death and differentiation, 16(11), 1493-1504 (2009-07-11)
The tumor suppressor p53 induces potent anti-proliferative responses in stressed cells; in unstressed cells this ability of p53 is restrained by Hdm2. Expression of Hdm2 is also induced by p53, thereby establishing feedback inhibition. Regulation of the p53-Hdm2 interaction and
Matthew Frieman et al.
Journal of virology, 83(13), 6689-6705 (2009-04-17)
The outcome of a viral infection is regulated in part by the complex coordination of viral and host interactions that compete for the control and optimization of virus replication. Severe acute respiratory syndrome coronavirus (SARS-CoV) intimately engages and regulates the

Protocols

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

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