Documents
F2442
Fetal Bovine Serum
USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma
Quality Level
biological source
bovine fetus
sterility
sterile-filtered
product line
FBS Classic
composition
Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL
origin
USA origin
application(s)
cell culture | hybridoma: suitable
cell culture | mammalian: suitable
impurities
≤10 EU/mL endotoxin
shipped in
dry ice
storage temp.
−20°C
Looking for similar products? Visit Product Comparison Guide
General description
Application
- perivascular-resident macrophage-like melanocytes (PVM/M) growth medium
- synthetic oviduct fluid (SOF)
- Eagle′s minimum essential medium (EMEM)
- neurobasal medium
- Hyclone McCoys 5 A medium
- Hanks medium
Biochem/physiol Actions
Linkage
Analysis Note
- Endotoxin and hemoglobin tested
- Tested for the presence of bacteria, virus, and mycoplasma
- Triple filtered with 0.1 micron membrane under aseptic conditions
Other Notes
Personal Protective Equipment
RIDADR
NONH for all modes of transport
WGK Germany
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
What is the level of growth factors in Fetal Bovine Serum, Product F2442?
Sigma Aldrich does not determine the levels of growth factors (such as TGF beta, VEGF, EGF, PDGF, or FGF) for serum with reduced levels of growth factors and plasma derived serum.
Is Fetal Bovine Serum, Product F2442, available as a charcoal stripped, de-lipidated serum?
We offer Product No. F6765 (Fetal Bovine Serum, charcoal stripped). The charcoal stripping decreases the levels of hormones; it may also decrease the levels of lipids. If further reduction of lipid levels is required, you may need to treat the serum with fumed silica (e.g. Product No. S5130). Use 20 grams of S5130 per Liter of serum (not plasma). It is mixed for 3-4 hours, then centrifuged in 1 L containers for about 15 minutes at 2000 xg. The supernatant can then be decanted, and filtered if desired. NOTE: Silica is extremely fluffy, and care must be taken not to inhale its dust. Allow for about 15% loss of the serum due to adsorption.
How is Fetal Bovine Serum, Product F2442, cell culture tested?
Biological performance is assessed using Sigma cell culture medium supplemented with the serum and tested at a final concentration of 10%. Seeding efficiency, doubling time, and final cell density are measured on appropriate cell lines. Growth studies are carried through two subculture generations and cell growth is plotted as a logarithmic function of time in culture. During the testing period, cultures are examined microscopically for atypical morphology and evidence of cytotoxicity. Hybridoma -tested serum has been shown to support mouse myeloma cells and hybridoma cells in culture.
What is the cloning assay performed on Fetal Bovine Serum, Product F2442?
The cloning efficiency assay is performed on each lot of FBS to verify that each lot will support clonal growth of myeloma cells and/or fusion products. The assay is performed using 5% and 10% concentrations of the FBS.
What is the difference between Fetal Bovine Serum F2442 and 12103C?
Product No. 12103C meets most of the specifications of F2442. Both products are from the same raw material suppliers and undergo the same stringent testing prior to release. However, the F2442 has been use-tested in hybridoma culture, whereas 12103C has not.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
Are all Fetal Bovine Serum (FBS) products heat inactivated?
Not all Fetal Bovine Serum products are heat inactivated. Product numbers F4135 and 12306C are heat-inactivated FBS.
There is a precipitate in my serum. Is my serum contaminated?
Precipitates in serum can often appear as large round bodies, even as doublets per microscopic exam. This can be mistaken as a yeast contamination. Brownian motion may be mistaken for motility. These are lipid aggregates that will be readily dissolved when exposed to lipase. Also, these particles will not gram stain or show any growth in culture on aerobic, anaerobic, or fungal media and culture conditions. The performance of the material is not effected. Centrifugation or filtration of small volumes will remove these particles.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical ServiceSupport
Customer SupportContact UsFAQSafety Data Sheets (SDS)Certificates (COA/COO)Quality & RegulatoryCalculators & AppsWebinarsSocial Media
MilliporeSigma
Research. Development. Production.
We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.
© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.
Reproduction of any materials from the site is strictly forbidden without permission.