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MilliporeSigma

F2442

Sigma-Aldrich

Fetal Bovine Serum

USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

Fetal Bovine Serum
1 of 1 reviewers took part in a promotion

Synonym(s):

Fetal Bovine Sera, Fetal Calf Serum, FBS, FCS, sera, serum

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About This Item

MDL number:
MFCD00132239
UNSPSC Code:
12352207
eCl@ss:
32160409
NACRES:
NA.71
Biological source:
bovine fetus
Origin:
USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice

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biological source

bovine fetus

Quality Level

500

sterility

sterile-filtered

product line

FBS Classic

composition

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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1 of 4

This Item
12103CF4135F8067
Fetal Bovine Serum USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

F2442

Fetal Bovine Serum

Fetal Bovine Serum USA origin, suitable for cell culture

12103C

Fetal Bovine Serum

Fetal Bovine Serum USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma

F4135

Fetal Bovine Serum

Fetal Bovine Serum New Zealand origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

F8067

Fetal Bovine Serum

origin

USA origin

origin

USA origin

origin

USA origin

origin

New Zealand origin

sterility

sterile-filtered

sterility

-

sterility

sterile-filtered

sterility

sterile-filtered

biological source

bovine fetus

biological source

bovine fetus

biological source

bovine fetus

biological source

bovine fetus

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | insect: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

impurities

≤10 EU/mL endotoxin

impurities

≤10 EU/mL endotoxin

impurities

≤10 EU/mL endotoxin

impurities

≤10 EU/mL endotoxin

General description

Fetal Bovine Serum (FBS) is a complex mixture with low and high molecular weight biomolecules exhibiting optimal growth-enhancing and growth-suppressing functions. FBS contains molecules, such as growth factors, proteins, vitamins, trace elements, hormones etc. These molecules are important for the growth and maintenance of cells. It is a common supplement for cell culture media.[1][2] FBS is derived from the calf blood. It is devoid of fibrin and clotting factors. Heat inactivation of FBS blocks the complement system.[3] FBS derived from clotted blood is the most widely used undefined supplement in eukaryotic, especially mammalian, cell culture.[4][5] It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM),[6] Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM),[7] M199[8] and Roswell Park Memorial Institute medium, RPMI-1640.[6]

Application

Fetal Bovine Serum has also been used as a component of:
  • perivascular-resident macrophage-like melanocytes (PVM/M) growth medium[9]
  • synthetic oviduct fluid (SOF)[10]
  • Eagle′s minimum essential medium (EMEM)[11]
  • neurobasal medium[12]
  • Hyclone McCoys 5 A medium[13]
  • Hanks medium[14]

Biochem/physiol Actions

FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Other Notes

Tested for the presence of bacteria, virus, mycoplasma, endotoxin and for hemoglobin content. Triple filtered with 0.1 micron membrane under aseptic conditions.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
24B125
25A064
24E021
24B458
24B128

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Questions

1–10 of 15 Questions  

  1. How to heat inactivate this serum Fetal Bovine Serum USA origin,(Cat Log - F2442)

    1 answer

    1. Heat inactivation occurs when serum is heated to 56°C for a period of 30 minutes.

      Here's a protocol for heat inactivation:
      1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.
      2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.
      3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.
      4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.

      Helpful?

  2. is this Heat Inactivate FBS?

    1 answer

    1. This product has not been heat inactivated.

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  3. can F2442 FBS be used for culturing peritoneal macrophages? what is the suitable one?

    1 answer

    1. This product is tested for suitability in mammalian cell culture. While it is not specifically tested on macrophages, it is subject to stringent specifications, including low endotoxin, and should perform well. Please see the link below to review a sample Certificate of Analysis:
      https://www.sigmaaldrich.com/coa/SIGMA/F2442/23A317

      Helpful?

  4. Can this f2442 fbs be used for culture expansion of mesenchymal stem cells? Please confirm Thanks

    1 answer

    1. Fetal Bovine Serum (Cat. no.: F2442) is suitable for use in mammalian cell culture. However, it is not tested specifically in mesenchymal stem cell culture. There is some evidence in the literature that this product would be suitable. See the link below for a relevant reference:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4545441/

      Helpful?

  5. There is a precipitate in my serum.  Is my serum contaminated?

    1 answer

    1. Precipitates in serum can often appear as large round bodies, even as doublets per microscopic exam. This can be mistaken as a yeast contamination. Brownian motion may be mistaken for motility. These are lipid aggregates that will be readily dissolved when exposed to lipase. Also, these particles will not gram stain or show any growth in culture on aerobic, anaerobic, or fungal media and culture conditions. The performance of the material is not effected. Centrifugation or filtration of small volumes will remove these particles.

      Helpful?

  6. Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?

    1 answer

    1. Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored  refrigerated at 2-8°C.  If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days.  For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance.  For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C.  For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.

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  7. How is Fetal Bovine Serum, Product F2442, cell culture tested?

    1 answer

    1. Biological performance is assessed using Sigma cell culture medium supplemented with the serum and tested at a final concentration of 10%. Seeding efficiency, doubling time, and final cell density are measured on appropriate cell lines. Growth studies are carried through two subculture generations and cell growth is plotted as a logarithmic function of time in culture. During the testing period, cultures are examined microscopically for atypical morphology and evidence of cytotoxicity. Hybridoma -tested serum has been shown to support mouse myeloma cells and hybridoma cells in culture.

      Helpful?

  8. Does Sigma-Aldrich offer delipidated bovine serum?

    1 answer

    1. At this time, Sigma-Aldrich does not offer delipidated bovine serum (either FBS or calf serum).A method for lipid depletion, though not necessarily that of Sigma:1. While mixing, gradually add 10 g fumed silica (Product S5130 or Aerosil) per liter of serum2. Mix at room temperature and then transfer to 2-8 °C for overnight mixing.3. After overnight mixing, remove flumed silica (Aerosil) by centrifugation at 3000-5000 rpm for 5 minutes.4. Retain the supernatant. The lipids should stick to the silica. There may be some protein loss

      Helpful?

  9. Does Sigma-Aldrich offer fetal bovine serum (FBS) that is antibiotic-free?

    1 answer

    1. FBS can be tested for the presence of antibiotics. Products F2442, F0926, and F6178, Fetal Bovine Serum are routinely tested for Tetracycline (by far the most commonly used antibiotic in animals) and the results are reported on the lot specific Certificate of Analysis.Per the USDA, Antibiotics may be given to prevent or treat disease in cattle. A "withdrawal" period is required from the time antibiotics are administered until it is legal to slaughter the animal. This is so residues can exit the animal's system. FSIS randomly samples cattle at slaughter and tests for residues. Data from this Monitoring Plan have shown a very low percentage of residue violations.The USDA does random sampling of all cattle submitted for slaughter in USDA inspected facilities for antibiotics. The mandatory withdrawal period is 24 hours. Based on withdrawal periods required by the USDA, animal testing at slaughter and finished product testing, our serum is antibiotic free.

      Helpful?

  10. Are all Fetal Bovine Serum (FBS) products heat inactivated?

    1 answer

    1. Not all Fetal Bovine Serum products are heat inactivated. Product numbers F4135 and 12306C are heat-inactivated FBS.

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1–10 of 15 Questions  

Reviews

1 of 1 reviewers took part in a promotion

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  1. 대한민국 서울
    • Review 1
    • Votes 4
    5 out of 5 stars.

    최고의 갓성비 FBS!

    정기배송으로 진행시 가격도 50만원으로 저렴하고 정해진 날짜에 와서 좋아요!
    DMSO같이 많이 쓰는 제품도 media와 같이 묶어서 사용하면 더 저렴해지더라구요 ㅎ

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