MilliporeSigma
All Photos(1)

Documents

F2637

Sigma-Aldrich

Ficoll® 400

BioXtra, for molecular biology, lyophilized powder

Sign Into View Organizational & Contract Pricing

Synonym(s):
Polysucrose 400, Ficoll 400
CAS Number:
MDL number:
NACRES:
NA.52

biological source

non-animal source

Quality Level

grade

Molecular Biology
for molecular biology

product line

BioXtra

form

lyophilized powder

foreign activity

DNase (Exonuclease), NICKase (Endonuclease), RNase and Protease, none detected

storage temp.

room temp

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
F8016F4375F5415
Sigma-Aldrich

F2637

Ficoll® 400

Ficoll® 400 lyophilized powder, Type 400-DL, BioReagent, suitable for cell culture

F8016

Ficoll® 400

Sigma-Aldrich

F4375

Ficoll® PM 400

Ficoll® solution Type 400, 20% in H2O

F5415

Ficoll® solution

biological source

non-animal source

biological source

-

biological source

-

biological source

-

form

lyophilized powder

form

-

form

powder, spray-dried

form

liquid

storage temp.

room temp

storage temp.

-

storage temp.

room temp

storage temp.

2-8°C

product line

BioXtra

product line

BioReagent

product line

-

product line

-

foreign activity

DNase (Exonuclease), NICKase (Endonuclease), RNase and Protease, none detected

foreign activity

-

foreign activity

-

foreign activity

-

General description

Ficoll® 400 is a non-ionic synthetic polymer of sucrose used for cell separation and organ isolation.

Application

Suitable for
  • hybridization experiments to reduce non-specific binding of labeled probes to the membrane
  • preparation of DNA loading buffer (15% Ficoll®, 0.25% bromophenol blue and 0.25% xylene cyanol FF
  • separation of cells with similar density but different size (PBMC from human peripheral blood, bone marrow and umbilical cord blood)
  • producing density gradients to separate subcellular components
  • vitrification of cell and tissue samples.
  • to compare the effect of different perfusates on Hypoxic pulmonary vasoconstriction (HPV).
Used for cell separation and organelle isolation.

Features and Benefits

  • free from DNases, RNases, phosphatases and proteases
  • preserves functional and morphological integrity of the separated cells

Other Notes

A nonionic synthetic polymer of sucrose.

Legal Information

Ficoll is a registered trademark of Cytiva

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 8

1 of 8

Ficoll® PM 400 Cytiva 17-0300-10, pack of 100 g

GE17-0300-10

Ficoll® PM 400

Ficoll® PM 400 Cytiva 17-0300-50, pack of 500 g

GE17-0300-50

Ficoll® PM 400

Ficoll® Paque Plus Cytiva 17-1440-02, pack of 6 × 100 mL

GE17-1440-02

Ficoll® Paque Plus

Histopaque®-1077 sterile-filtered, density: 1.077 g/mL

Sigma-Aldrich

10771

Histopaque®-1077

Ficoll®-Paque Premium Cytiva 17-5442-02, pack of 6 × 100 mL

GE17-5442-02

Ficoll®-Paque Premium

Ficoll® PM 70 Cytiva 17-0310-50, pack of 500 g

GE17-0310-50

Ficoll® PM 70

Hae Young Yoo et al.
Pulmonary circulation, 3(2), 396-405 (2013-09-10)
Hypoxic pulmonary vasoconstriction (HPV) is a compensatory physiological mechanism in the lung that optimizes the matching of ventilation to perfusion and thereby maximizes gas exchange. Historically, HPV has been primarily studied in isolated perfused/ventilated lungs; however, the results of these
Ivana Gessara et al.
Stem cell reports, 16(4), 784-796 (2021-03-20)
The ability to genetically manipulate organisms has led to significant insights into functional genomics in many species. In birds, manipulation of the genome is hindered by the inaccessibility of the one-cell embryo. During embryonic development, avian primordial germ cells (PGCs)
Gaye Cetinkaya et al.
Cryobiology, 63(3), 292-297 (2011-10-25)
Preservation of cell and tissue samples from endangered species is a part of biodiversity conservation strategy. Therefore, setting up proper cell and tissue cryopreservation methods is very important as these tissue samples and cells could be used to reintroduce the
Cristina Batlle et al.
Cell reports, 30(4), 1117-1128 (2020-01-30)
Prion-like proteins form multivalent assemblies and phase separate into membraneless organelles. Heterogeneous ribonucleoprotein D-like (hnRNPDL) is a RNA-processing prion-like protein with three alternative splicing (AS) isoforms, which lack none, one, or both of its two disordered domains. It has been
Zhaoming Yang et al.
Frontiers in endocrinology, 11, 587450-587450 (2020-12-29)
Islet culture prior to transplantation is a standard practice in many transplantation centers. Nevertheless, the abundant islet mass loss and function impairment during this serum-deprivation culture period restrain the success of islet transplantation. In the present study, we used a

Articles

Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.

Protocols

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service