F2772

Sigma-Aldrich

Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody produced in goat

flow cytometry grade, affinity isolated antibody, buffered aqueous solution

MDL number:
NACRES:
NA.46

Quality Level

biological source

goat

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

grade

flow cytometry grade

clone

polyclonal

form

buffered aqueous solution

storage condition

protect from light

application(s)

flow cytometry: 1:100
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160

conjugate

FITC conjugate

storage temp.

−20°C

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General description

Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Anti-mouse IgG is conjugated to Fluorescein Isothiocyanate (FITC), Isomer I. The F(ab′)2 antibodies isolated from goat antiserum by affinity chromatography, react specifically with mouse IgG Fc; does not bind other mouse Igs. No cross reaction is observed with mouse Fab fragment. The antibody shows minimal cross reaction with human, horse and bovine proteins on tissue or cell preparations.
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids.
Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.

Specificity

Goat polyclonal anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody isolated from goat antiserum by affinity chromatography reacts specifically with mouse Fc fragment. No cross reaction is observed with mouse Fab fragment. The antibodies are adsorbed to assure minimal cross reaction with human, horse and bovine proteins on tissue or cell preparations.

Immunogen

Purified Fc fragments of mouse IgG

Application

Goat polyclonal anti-Mouse IgG (Fc specific) F(ab′)2 fragment-FITC antibody may be used as a reagent in flow cytometry, immunohistology and immunocytology offering sensitive and specific activity to mouse IgG and no cross reactivity with human, horse or bovine immunoglobulins.
Immunohistology: A minimum dilution of 1:160 is determined by indirect immunofluorescence on formalinfixed, and paraffin-embedded human tonsil using mouse monoclonal anti-human IgG as the primary antibody.
Anti-Mouse IgG (Fc specific) F(ab′)2 fragment-FITC antibody produced in goat has been used in :
  • flow cytometry for labelling of human peripheral blood lymphocytes (dilution of 1:100) and label human polymorphonuclear cells (PMNs) (dilution of 1:50).
  • immunocytochemistry using human embryonic stem cells
  • immunohistochemistry at a working dilution of 1:160.

Biochem/physiol Actions

immunoglobulin G (IgG) antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgG antibody participates in complement fixation and opsonization.

Other Notes

Antibody adsorbed with bovine, equine and human serum proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background with bovine, horse or human samples.
Useful when trying to avoid background staining due to the presence of Fc receptors.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Raheem Peerani et al.
Methods in molecular biology (Clifton, N.J.), 482, 21-33 (2008-12-18)
Local micro-environmental cues consisting of soluble cytokines, extra-cellular matrix (ECM), and cell-cell contacts are determining factors in stem cell fate. These extrinsic cues form a 'niche' that governs a stem cell's decision to either self-renew or differentiate into one or...
J S Plested et al.
Infection and immunity, 69(5), 3203-3213 (2001-04-09)
A recently described flow cytometric opsonophagocytic assay (OPA) was adapted to quantify the functional activity of serum antibodies specifically directed against serogroup B inner core lipopolysaccharide (LPS) of Neisseria meningitidis. The percentage of human peripheral polymorphonuclear leukocytes and monocytes (PMNms)...
Pernille B Jørgensen et al.
PloS one, 7(9), e46120-e46120 (2012-10-11)
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M Kita-Furuyama et al.
Clinical and experimental immunology, 131(2), 234-240 (2003-02-04)
Dendritic cells (DCs) are the most potent antigen-presenting cells and a prerequisite for the initiation of primary immune response. This study was performed to investigate the contribution of DCs to the initiation of Graves' hyperthyroidism, an organ-specific autoimmune disease in...
Morten Harboe et al.
Journal of immunology (Baltimore, Md. : 1950), 189(5), 2606-2613 (2012-08-02)
Properdin is well known as an enhancer of the alternative complement amplification loop when C3 is activated, whereas its role as a recognition molecule of exogenous pathogen-associated molecular patterns and initiator of complement activation is less understood. We therefore studied...

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