F2878

Sigma-Aldrich

Ficoll® PM 70

Type 70

Synonym(s):
Poly(sucrose-co-epichlorhydrin)
CAS Number:
MDL number:
NACRES:
NA.25

type

Type 70

Quality Level

form

powder

mol wt

~70,000

solubility

H2O: soluble 100 mg/mL, clear to slightly hazy, colorless to faintly yellow

Looking for similar products? Visit Product Comparison Guide

Application

Ficoll® PM 70 is used as a macromolecular crowding agent in studies of cell volume signaling and protein refolding. It may be used in tissue engineering and macromolecular conformation research for the development, evaluation and use of macromolecular crowding (MMC) systems and configurations. Ficoll PM-70 may be used in vitrification research.

Other Notes

A nonionic synthetic polymer of sucrose.

Legal Information

Ficoll is a registered trademark of Cytiva

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Emmanuel Dauty et al.
Journal of molecular recognition : JMR, 17(5), 441-447 (2004-09-14)
Aqueous environments in living cells are crowded, with up to >50 wt% small and macromolecule-size solutes. We investigated quantitatively one important consequence of molecular crowding--reduced diffusion of biologically important solutes. Fluorescence correlation spectroscopy (FCS) was used to measure the diffusion...
Nobuhiko Tokuriki et al.
Protein science : a publication of the Protein Society, 13(1), 125-133 (2003-12-24)
Unfolded states of ribonuclease A were used to investigate the effects of macromolecular crowding on macromolecular compactness and protein folding. The extent of protein folding and compactness were measured by circular dichroism spectroscopy, fluorescence correlation spectroscopy, and NMR spectroscopy in...
M B Burg
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 10(5-6), 251-256 (2000-12-23)
The non-ideal properties of solutions containing high concentrations of macromolecules can result in enormous increases in the activity of the individual macromolecules. There is considerable evidence that macromolecular crowding and confinement not only occur in cells, but that these are...
Y Georgalis et al.
Journal of colloid and interface science, 386(1), 141-147 (2012-08-23)
Ficoll PM70 is an important branched polysaccharide polymer with a variety of applications in biology and biophysics. The accepted picture for the aqueous solutions of this polymer is that of a polydisperse nearly spherical suspension with a molecular weight of...
M Al-Habori
The international journal of biochemistry & cell biology, 33(9), 844-864 (2001-07-20)
Macromolecular crowding has been proposed as a mechanism by means of which a cell can sense relatively small changes in volume or, more accurately, the concentration of intracellular solutes. According to the macromolecular theory, the kinetics and equilibria of enzymes...
Related Content
Ficoll 400 is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400 is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400 is very hydrophilic and extremely water-soluble.
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service