F3290

FLAG^® Peptide

lyophilized powder

• Synonym(s): Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, ddddk peptide, dykddddk peptide
• Empirical Formula (Hill Notation): C₄₁H₆₀N₁₀O₂₀
• Molecular Weight: 1012.97
• MDL number: MFCD02262135
• PubChem Substance ID: 329799698
• NACRES: NA.32

Properties

• form: lyophilized powder
• Quality Level: 200
• mol wt: 1012.97 Da
• shipped in: wet ice
• storage temp.: 2-8°C
• SMILES string: NCCCC[C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@@H](N)CC(O)=O)C(O)=O
• InChI: 1S/C41H60N10O20/c42-11-3-1-5-22(45-36(65)24(13-19-7-9-20(52)10-8-19)47-34(63)21(44)14-29(53)54)35(64)48-26(16-31(57)58)38(67)50-28(18-33(61)62)40(69)51-27(17-32(59)60)39(68)49-25(15-30(55)56)37(66)46-23(41(70)71)6-2-4-12-43/h7-10,21-28,52H,1-6,11-18,42-44H2,(H,45,65)(H,46,66)(H,47,63)(H,48,64)(H,49,68)(H,50,67)(H,51,69)(H,53,54)(H,55,56)(H,57,58)(H,59,60)(H,61,62)(H,70,71)/t21-,22-,23-,24-,25-,26-,27-,28-/m0/s1
• InChI key: XZWYTXMRWQJBGX-VXBMVYAYSA-N

Description

General description

The FLAG peptide is used for the competitive elution of amino-terminal, Met-amino-terminal or carboxy-terminal FLAG fusion proteins from the Anti-FLAG M1 or Anti-FLAG M2 antibody (in solution or bound to an agarose gel).

Application

Gently elutes FLAG fusion proteins from ANTI-FLAG^® M1 and M2 affinity resins. Usual working concentration is 100 μg/ml. Will not elute 3X FLAG fusion proteins. For this application, use 3X FLAG Peptide, F4799.

Browse additional application references in our FLAG^® Literature portal.

Preparation Note

For stock solution, dissolve in TBS (10mM Tris HCL, 150 mM NaCl, pH7.4) to a final concentration of 5 mg/mL.

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves