F3380

Sigma-Aldrich

Factor V Activating Enzyme from Russell's viper venom

lyophilized powder, 20 units/mg protein

CAS Number:
Enzyme Commission number:
MDL number:

form

lyophilized powder

Quality Level

specific activity

20 units/mg protein

storage temp.

−20°C

Application

Factor V activating enzyme from Russell′s viper venom (RVV) is a single-chain glycoprotein that is involved in the rapid clotting of blood. Factor V circulates in the blood as an inactive cofactor and must be activated by proteases such as Factor V activating enzyme from RVV . This product may be useful in studying the blood coagulation cascade as well as the inherited deficiency called parahemophilia .

Biochem/physiol Actions

Factor V activator for RVV contains fucose, mannose, galactose, glucosamine, and neuraminic acid. Factor V activating enzyme from RVV is an arginine esterase that is sensitive to diisopropyl fluorophosphate (DFP) .

Unit Definition

One unit will increase the Factor V activity in 0.1 mL of fresh normal human plasma by at least 2× after incubation for 3 min at 37 °C.

Physical form

Stabilized in albumin and sodium chloride.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

The C-terminus of tissue factor pathway inhibitor α is required for its interaction with factors V and Va.
M Ndonwi et al.
Journal of thrombosis and haemostasis : JTH, 10(9), 1944-1946 (2012-06-29)
Maria Cristina Bravo et al.
BMC systems biology, 6, 45-45 (2012-05-23)
Because understanding of the inventory, connectivity and dynamics of the components characterizing the process of coagulation is relatively mature, it has become an attractive target for physiochemical modeling. Such models can potentially improve the design of therapeutics. The prothrombinase complex...
Daisuke Nakayama et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 65(Pt 12), 1306-1308 (2010-01-08)
Russell's viper venom blood coagulation factor V activator (RVV-V) is a thrombin-like serine proteinase that specifically activates factor V by cleaving a single peptide bond between Arg1545 and Ser1546. Activated factor V combines with activated factor X produced by the...
Kenneth Segers et al.
The Journal of biological chemistry, 282(47), 33915-33924 (2007-09-20)
Human blood coagulation Factor V (FV) is a plasma protein with little procoagulant activity. Limited proteolysis at Arg(709), Arg(1018), and Arg(1545) by thrombin or Factor Xa (FXa) results in the generation of activated FV, which serves as a cofactor of...
Snake venom proteases that activate blood-coagulation factor V.
W Kisiel et al.
Methods in enzymology, 80 Pt C, 275-285 (1981-01-01)

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