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F3380

Sigma-Aldrich

Factor V Activating Enzyme from Russell's viper venom

lyophilized powder, 20 units/mg protein

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CAS Number:
Enzyme Commission number:
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form

lyophilized powder

Quality Level

specific activity

20 units/mg protein

storage temp.

−20°C

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This Item
V2501P6556P2308
specific activity

20 units/mg protein

specific activity

-

specific activity

≥30 units/mg protein

specific activity

≥30 units/mg protein

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

400

Application

Factor V activating enzyme from Russell′s viper venom (RVV) is a single-chain glycoprotein that is involved in the rapid clotting of blood. Factor V circulates in the blood as an inactive cofactor and must be activated by proteases such as Factor V activating enzyme from RVV . This product may be useful in studying the blood coagulation cascade as well as the inherited deficiency called parahemophilia .

Biochem/physiol Actions

Factor V activator for RVV contains fucose, mannose, galactose, glucosamine, and neuraminic acid. Factor V activating enzyme from RVV is an arginine esterase that is sensitive to diisopropyl fluorophosphate (DFP) .

Unit Definition

One unit will increase the Factor V activity in 0.1 mL of fresh normal human plasma by at least 2× after incubation for 3 min at 37 °C.

Physical form

Stabilized in albumin and sodium chloride.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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F Tokunaga et al.
The Journal of biological chemistry, 263(33), 17471-17481 (1988-11-25)
The complete amino acid sequences of two isoproteins of the factor V-activating enzyme (RVV-V) isolated from Vipera russelli (Russell's viper) venom were determined by sequencing S-pyridylethylated derivatives of the proteins and their peptide fragments generated by either chemical (cyanogen bromide
Kenneth Segers et al.
Proteins, 64(4), 968-984 (2006-06-30)
Blood coagulation factor V (FV) is a multifunctional protein that circulates in human plasma as a precursor molecule which can be activated by thrombin or activated factor X (FXa) in order to express its cofactor activity in prothrombin activation. FV
Bioengineered factor Xa as a potential new strategy for hemophilia therapy.
Rodney M Camire
Expert review of hematology, 5(2), 121-123 (2012-04-06)
W Kisiel
The Journal of biological chemistry, 254(23), 12230-12234 (1979-12-10)
The protease from Russell's viper venom that activates Factor V was purified by gel filtration on Sephadex G-150 and ion exchange column chromatography on sulfopropyl (SP)-Sephadex C-50. The purified enzyme is a glycoprotein containing 6% carbohydrate. It migrated as a
E Siigur et al.
Biochimica et biophysica acta, 1429(1), 239-248 (1999-01-27)
A factor V activator (VLFVA) was separated from Vipera lebetina venom by gel filtration on Sephadex G-100 superfine, followed by chromatography on CM-cellulose and on heparin-agarose. This enzyme (VLFVA) with a molecular mass of 28.4 kDa, as determined by matrix

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