F3380

Sigma-Aldrich

Factor V Activating Enzyme from Russell's viper venom

lyophilized powder, 20 units/mg protein

CAS Number:
Enzyme Commission number:
MDL number:

form

lyophilized powder

Quality Level

specific activity

20 units/mg protein

storage temp.

−20°C

Application

Factor V activating enzyme from Russell′s viper venom (RVV) is a single-chain glycoprotein that is involved in the rapid clotting of blood. Factor V circulates in the blood as an inactive cofactor and must be activated by proteases such as Factor V activating enzyme from RVV . This product may be useful in studying the blood coagulation cascade as well as the inherited deficiency called parahemophilia .

Biochem/physiol Actions

Factor V activator for RVV contains fucose, mannose, galactose, glucosamine, and neuraminic acid. Factor V activating enzyme from RVV is an arginine esterase that is sensitive to diisopropyl fluorophosphate (DFP) .

Unit Definition

One unit will increase the Factor V activity in 0.1 mL of fresh normal human plasma by at least 2× after incubation for 3 min at 37 °C.

Physical form

Stabilized in albumin and sodium chloride.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Kenneth Segers et al.
Proteins, 64(4), 968-984 (2006-06-30)
Blood coagulation factor V (FV) is a multifunctional protein that circulates in human plasma as a precursor molecule which can be activated by thrombin or activated factor X (FXa) in order to express its cofactor activity in prothrombin activation. FV...
Laura M Haynes et al.
Arteriosclerosis, thrombosis, and vascular biology, 32(12), 2877-2883 (2012-10-02)
Rivaroxaban is an oral anticoagulant that directly targets both free factor Xa and factor Xa in complex with its protein cofactor, factor Va, in the prothrombinase complex. It is approved in the United States for the prophylaxis of deep vein...
Blood coagulation factors V and VIII: structural and functional similarities and their relationship to hemorrhagic and thrombotic disorders.
W H Kane et al.
Blood, 71(3), 539-555 (1988-03-01)
Rinku Majumder et al.
The Journal of biological chemistry, 286(41), 35535-35542 (2011-08-24)
Constituents of platelet membranes regulate the activity of the prothrombinase complex. We demonstrate that membranes containing phosphatidylcholine and phosphatidylethanolamine (PE) bind factor Va with high affinity (K(d) = ∼10 nm) in the absence of phosphatidylserine (PS). These membranes support formation...
F Tokunaga et al.
The Journal of biological chemistry, 263(33), 17471-17481 (1988-11-25)
The complete amino acid sequences of two isoproteins of the factor V-activating enzyme (RVV-V) isolated from Vipera russelli (Russell's viper) venom were determined by sequencing S-pyridylethylated derivatives of the proteins and their peptide fragments generated by either chemical (cyanogen bromide...

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